Cells infected with viruses and fixed when viral antigens appeared at the cell membrane induced much higher alpha interferon (IFN-α) levels in human peripheral blood mononuclear cells (PBMC) than free virions. Relatively few inducer cells were sufficient for triggering IFN production. Optimal IFN yields depended on inducer/producer cell ratio. The response was peculiar to PBMC as it was not found in other cells in which IFN can normally be induced by free virions. IFN inducing activity was also exerted by live virus-infected PBMC, showing that this type of induction may have physiological importance. These findings confirm that viral induction of IFN-α is activated by some interaction between viral components presented at the cell surface and PBMC membrane. Thus induction of IFN by circulating cells infected by viruses and presenting viral antigens at the surface may be an efficient host defense mechanism. Since IFN yields close to 106 international units per millimeter are obtained, this system has potential for large scale production of native IFN-α.

Induction of alpha interferon by membrane interaction between viral surface and peripheral blood mononuclear cells / M. R., Capobianchi; J., Facchini; DI MARCO, Paola; Antonelli, Guido; F., Dianzani. - In: PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE. - ISSN 0037-9727. - STAMPA. - 178:4(1985), pp. 551-556.

Induction of alpha interferon by membrane interaction between viral surface and peripheral blood mononuclear cells

DI MARCO, Paola;ANTONELLI, Guido;
1985

Abstract

Cells infected with viruses and fixed when viral antigens appeared at the cell membrane induced much higher alpha interferon (IFN-α) levels in human peripheral blood mononuclear cells (PBMC) than free virions. Relatively few inducer cells were sufficient for triggering IFN production. Optimal IFN yields depended on inducer/producer cell ratio. The response was peculiar to PBMC as it was not found in other cells in which IFN can normally be induced by free virions. IFN inducing activity was also exerted by live virus-infected PBMC, showing that this type of induction may have physiological importance. These findings confirm that viral induction of IFN-α is activated by some interaction between viral components presented at the cell surface and PBMC membrane. Thus induction of IFN by circulating cells infected by viruses and presenting viral antigens at the surface may be an efficient host defense mechanism. Since IFN yields close to 106 international units per millimeter are obtained, this system has potential for large scale production of native IFN-α.
1985
01 Pubblicazione su rivista::01a Articolo in rivista
Induction of alpha interferon by membrane interaction between viral surface and peripheral blood mononuclear cells / M. R., Capobianchi; J., Facchini; DI MARCO, Paola; Antonelli, Guido; F., Dianzani. - In: PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE. - ISSN 0037-9727. - STAMPA. - 178:4(1985), pp. 551-556.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/452165
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