Abstract This study has been aimed at improving some steps in the gas-liquid chromatographic determination of sulfated bile acids. The best conditions for the enzymatic hydrolysis with cholylglycine hydrolase of sulfolithocholylglycine and sulfolithocholyltaurine are described. Recoveries of more than 85% were obtained after prolonging the incubation to 12 h. A single-step procedure for solvolysis and methylation of bile acids was achieved by using, as a water scavenger, 2,2-dimethoxypropane added directly to the hydrolysis mixture. This procedure avoided the difficulty of the extraction of sulfated bile acids from aqueous solutions.
An improved gas-chromatographic method for the determination of sulfated and unsulfated bile acids in serum / A., Cantafora; Angelico, Mario; Attili, Adolfo Francesco; L., Ercoli; Capocaccia, Livio. - In: CLINICA CHIMICA ACTA. - ISSN 0009-8981. - STAMPA. - 95:3(1979), pp. 501-508.
An improved gas-chromatographic method for the determination of sulfated and unsulfated bile acids in serum
ANGELICO, Mario;ATTILI, Adolfo Francesco;CAPOCACCIA, Livio
1979
Abstract
Abstract This study has been aimed at improving some steps in the gas-liquid chromatographic determination of sulfated bile acids. The best conditions for the enzymatic hydrolysis with cholylglycine hydrolase of sulfolithocholylglycine and sulfolithocholyltaurine are described. Recoveries of more than 85% were obtained after prolonging the incubation to 12 h. A single-step procedure for solvolysis and methylation of bile acids was achieved by using, as a water scavenger, 2,2-dimethoxypropane added directly to the hydrolysis mixture. This procedure avoided the difficulty of the extraction of sulfated bile acids from aqueous solutions.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
