A histological study ofin vitro cultured cotyledonary expiants of tomato (Lycopersicon esculentum) was performed in order to determine the site (differentiated tissue or developing callus) and the mode of plant regeneration. Results have shown that callus develops at the excision sites of cotyledonary expiants and that shoots are formed exclusively within the unorganized callus: excision areas are the only morphogenetic sites and the proximal excision is the preferred site for plant regeneration. Shoots differentiate by organogenesis within the superficial region of the callus. Few neocambial cells cooperate in the neoformation. Origin from a single cell is highly unlikely since rarely observed single activated cells never developed into shoots. Regenerated plants may be chimeras if invitro culture induces genetic diversity in the initial cells.
THE HISTOGENESIS OF SOMACLONES FROM TOMATO (LYCOPERSICON-ESCULENTUM MILL) COTYLEDONS / Monacelli, Barbara; Altamura, Maria Maddalena; Pasqua, Gabriella; M. G., Biasini; F., Sala. - In: PROTOPLASMA. - ISSN 0033-183X. - ELETTRONICO. - 142:2-3(1988), pp. 156-163. [10.1007/bf01290872]
THE HISTOGENESIS OF SOMACLONES FROM TOMATO (LYCOPERSICON-ESCULENTUM MILL) COTYLEDONS
MONACELLI, Barbara;ALTAMURA, Maria Maddalena;PASQUA, Gabriella;
1988
Abstract
A histological study ofin vitro cultured cotyledonary expiants of tomato (Lycopersicon esculentum) was performed in order to determine the site (differentiated tissue or developing callus) and the mode of plant regeneration. Results have shown that callus develops at the excision sites of cotyledonary expiants and that shoots are formed exclusively within the unorganized callus: excision areas are the only morphogenetic sites and the proximal excision is the preferred site for plant regeneration. Shoots differentiate by organogenesis within the superficial region of the callus. Few neocambial cells cooperate in the neoformation. Origin from a single cell is highly unlikely since rarely observed single activated cells never developed into shoots. Regenerated plants may be chimeras if invitro culture induces genetic diversity in the initial cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
