Red blood cell S-adenosylhomocysteine hydrolase (AHCY) from individuals of 1, 2-1 and 3-1 phenotypes was partially purified and K(m) and V(max) determined in the absence and in the presence of the following inhibitors: 3-deaza-adenosine (DZA), 3-deaza-aristeromycin (DZAry), 2-chloro adenosine (2-Cl-ado) and purine riboside (or nebularine). The three phenotypes 1, 2-1, 3-1 showed similar K(m) (32.58, 39.22 and 34.84μM respectively), but the ratio K(m)/V(max) was statistically different. DZA and DZAry appeared to be strong competitive inhibitors. The AHCY 1 phenotype was more resistant to their action, while the 3-1 variant was more sensitive. 2-Cl-ado and purine riboside were weaker inhibitors; the type of inhibition varied among the three phenotypes, but, again, the AHCY 1 phenotype was less sensitive than the other two.
Kinetic properties of the common electrophoretic variants of human S-adenosylhomocysteine hydrolase (AHCY): The effect of four nucleoside analogue inhibitors / Corbo, Rosa Maria; R., Ingianna; R., Scacchi; A., Bozzi. - In: ANNALS OF HUMAN GENETICS. - ISSN 0003-4800. - STAMPA. - 56:1(1992), pp. 35-43. [10.1111/j.1469-1809.1992.tb01127.x]
Kinetic properties of the common electrophoretic variants of human S-adenosylhomocysteine hydrolase (AHCY): The effect of four nucleoside analogue inhibitors
CORBO, Rosa Maria;
1992
Abstract
Red blood cell S-adenosylhomocysteine hydrolase (AHCY) from individuals of 1, 2-1 and 3-1 phenotypes was partially purified and K(m) and V(max) determined in the absence and in the presence of the following inhibitors: 3-deaza-adenosine (DZA), 3-deaza-aristeromycin (DZAry), 2-chloro adenosine (2-Cl-ado) and purine riboside (or nebularine). The three phenotypes 1, 2-1, 3-1 showed similar K(m) (32.58, 39.22 and 34.84μM respectively), but the ratio K(m)/V(max) was statistically different. DZA and DZAry appeared to be strong competitive inhibitors. The AHCY 1 phenotype was more resistant to their action, while the 3-1 variant was more sensitive. 2-Cl-ado and purine riboside were weaker inhibitors; the type of inhibition varied among the three phenotypes, but, again, the AHCY 1 phenotype was less sensitive than the other two.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.