Use of Lipase Immobilized on Celluse Support for Cleaning Aged Oil Layers

The present study reports (i) the covalent immobilization of Candida rugosa lipase on different cellulose supports (cotton buds, make-up remover pads, cellulose powder, cotton and tissues) using sodium periodate as activating agent and (ii) its application on the aged linseed oil removal from canvas. The optimization of experimental conditions such as pH, temperature and reaction time was performed for both, immobilization procedure and the biocatalyst application. Thus optimal conditions of immobilization were pH 7.0, 20 degrees C, 0.3 mg lipase loading per mg support and 200 min reaction time, while those for treating canvas surface, having stratified aged linseed oil were pH 6, 40 degrees C and 45 min reaction time. The ability of the immobilized lipase to remove aged oil films was confirmed by UV-Vis spectroscopy, high performance liquid chromatography (HPLC) and scanning electron microscopic (SEM) analysis.