The study was performed to determine if there is an association between the genotype and transmission of HHV-8 types A and C. These HHV-8 subtypes are prevalent in the area of North of Sardinia, which is an island off west Italy's mainland that has a high HHV-8 seroprevalence (35%). Blood and saliva samples from 30 patients with classic Kaposi's sarcoma who were lifetime residents of North Sardinia were analyzed to identify the HHV-8 genotype and quantitate the viral load. Genotype A, especially A1 subtype, was found more frequently (9/30 patients) and had a significantly higher viral load in saliva compared to blood (P=0.029), where type C was found more frequently but with a viral load lower than 10 3copies/ml. To determine if there is a correlation between the viral genotype and cellular tropism, type A1 and C3 HHV-8 viral particles were obtained from cell lines BCBL1 and BC3 infected chronically with HHV-8 A1 and C3 genotypes respectively and used to infect HEK293 epithelial origin cells and PBMCs in vitro. The data indicate that the A1 HHV-8 genotype is tropic and replicates at higher levels in the epithelial cell lines. © 2012 Wiley Periodicals, Inc.

In vivo and in vitro evidence for an association between the route-specific transmission of HHV-8 and the virus genotype / Barbara, Matteoli; Francesco, Broccolo; Antonio, Scaccino; Francesca, Cottoni; Angeloni, Antonio; Faggioni, Alberto; Luca Ceccherini, Nelli. - In: JOURNAL OF MEDICAL VIROLOGY. - ISSN 0146-6615. - STAMPA. - 84:5(2012), pp. 786-791. [10.1002/jmv.23246]

In vivo and in vitro evidence for an association between the route-specific transmission of HHV-8 and the virus genotype

ANGELONI, Antonio;FAGGIONI, Alberto;
2012

Abstract

The study was performed to determine if there is an association between the genotype and transmission of HHV-8 types A and C. These HHV-8 subtypes are prevalent in the area of North of Sardinia, which is an island off west Italy's mainland that has a high HHV-8 seroprevalence (35%). Blood and saliva samples from 30 patients with classic Kaposi's sarcoma who were lifetime residents of North Sardinia were analyzed to identify the HHV-8 genotype and quantitate the viral load. Genotype A, especially A1 subtype, was found more frequently (9/30 patients) and had a significantly higher viral load in saliva compared to blood (P=0.029), where type C was found more frequently but with a viral load lower than 10 3copies/ml. To determine if there is a correlation between the viral genotype and cellular tropism, type A1 and C3 HHV-8 viral particles were obtained from cell lines BCBL1 and BC3 infected chronically with HHV-8 A1 and C3 genotypes respectively and used to infect HEK293 epithelial origin cells and PBMCs in vitro. The data indicate that the A1 HHV-8 genotype is tropic and replicates at higher levels in the epithelial cell lines. © 2012 Wiley Periodicals, Inc.
2012
classic ks; hhv-8; cellular tropism; transmission; hhv-8 genotype
01 Pubblicazione su rivista::01a Articolo in rivista
In vivo and in vitro evidence for an association between the route-specific transmission of HHV-8 and the virus genotype / Barbara, Matteoli; Francesco, Broccolo; Antonio, Scaccino; Francesca, Cottoni; Angeloni, Antonio; Faggioni, Alberto; Luca Ceccherini, Nelli. - In: JOURNAL OF MEDICAL VIROLOGY. - ISSN 0146-6615. - STAMPA. - 84:5(2012), pp. 786-791. [10.1002/jmv.23246]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/441046
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