Peptidic hydrogels are an interesting class of materials, with potential applications in tissue engineering and controlled drug delivery. Fmoc-Phe(3) self-assembling peptide was synthesized in an aqueous phase by using a lipase from Pseudomonas fluorescens. The bioconversion afforded a self-supporting hydrogel in only 10 minutes, a feature that seems very promising for biotechnological applications. The rheological properties of such material were studied. The peptide hydrogel, used as a scaffold for culturing rat microglial cells, induced a significant cell proliferation and an increased production of the neurotrophic factor NGF. Moreover, microscopy studies demonstrated that the hydrogel was able to promote microglial cell adhesion.

Self-assembling peptide hydrogels promote microglial cells proliferation and NGF production / Chronopoulou, Laura; Togna, Anna Rita; Giulia, Guarguaglini; Masci, Giancarlo; Francesca, Giammaruco; Togna, Giuseppina Ines; Palocci, Cleofe. - In: SOFT MATTER. - ISSN 1744-683X. - STAMPA. - 8:21(2012), pp. 5784-5790. [10.1039/c2sm25528f]

Self-assembling peptide hydrogels promote microglial cells proliferation and NGF production

CHRONOPOULOU, LAURA;TOGNA, Anna Rita;MASCI, Giancarlo;TOGNA, Giuseppina Ines;PALOCCI, Cleofe
2012

Abstract

Peptidic hydrogels are an interesting class of materials, with potential applications in tissue engineering and controlled drug delivery. Fmoc-Phe(3) self-assembling peptide was synthesized in an aqueous phase by using a lipase from Pseudomonas fluorescens. The bioconversion afforded a self-supporting hydrogel in only 10 minutes, a feature that seems very promising for biotechnological applications. The rheological properties of such material were studied. The peptide hydrogel, used as a scaffold for culturing rat microglial cells, induced a significant cell proliferation and an increased production of the neurotrophic factor NGF. Moreover, microscopy studies demonstrated that the hydrogel was able to promote microglial cell adhesion.
2012
peptides; hydrogels; cell proliferation
01 Pubblicazione su rivista::01a Articolo in rivista
Self-assembling peptide hydrogels promote microglial cells proliferation and NGF production / Chronopoulou, Laura; Togna, Anna Rita; Giulia, Guarguaglini; Masci, Giancarlo; Francesca, Giammaruco; Togna, Giuseppina Ines; Palocci, Cleofe. - In: SOFT MATTER. - ISSN 1744-683X. - STAMPA. - 8:21(2012), pp. 5784-5790. [10.1039/c2sm25528f]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/440354
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