Signal transducer and activator of transcription 2 (STAT2), the critical component of type I interferons signaling, is a prototype latent cytoplasmic signal-dependent transcription factor. Activated tyrosine-phosphorylated STAT2 associates with STAT1 and IRF9 to bind the ISRE elements in the promoters of a subset of IFN-inducible genes (ISGs). In addition to activate hundreds of ISGs, IFN alpha also represses numerous target genes but the mechanistic basis for this dual effect and transcriptional repression is largely unknown. We investigated by ChIP-chip the binding dynamics of STAT2 and "active" phospho(P)-STAT2 on 113 putative IFN alpha direct target promoters before and after IFN alpha induction in Huh7 cells and primary human hepatocytes (PHH). STAT2 is already bound to 62% of our target promoters, including most "classical" ISGs, before IFN alpha treatment. 31% of STAT2 basally bound promoters also show P-STAT2 positivity. By correlating in vivo promoter occupancy with gene expression and changes in histone methylation marks we found that: 1) STAT2 plays a role in regulating ISGs expression, independently from its phosphorylation; 2) P-STAT2 is involved in ISGs repression; 3) "activated" ISGs are marked by H3K4me1 and H3K4me3 before IFN alpha; 4) "repressed" genes are marked by H3K27me3 and histone methylation plays a dominant role in driving IFN alpha-mediated ISGs repression.

Chromatin dynamics of gene activation and repression in response to interferon alpha (IFN(alpha)) reveal new roles for phosphorylated and unphosphorylated forms of the transcription factor STAT2 / B., Testoni; C., Vollenkle; Guerrieri, Francesca; S., Gerbal Chaloin; G., Blandino; Levrero, Massimo. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 286:23(2011), pp. 20217-20227. [10.1074/jbc.m111.231068]

Chromatin dynamics of gene activation and repression in response to interferon alpha (IFN(alpha)) reveal new roles for phosphorylated and unphosphorylated forms of the transcription factor STAT2

GUERRIERI, Francesca;LEVRERO, Massimo
2011

Abstract

Signal transducer and activator of transcription 2 (STAT2), the critical component of type I interferons signaling, is a prototype latent cytoplasmic signal-dependent transcription factor. Activated tyrosine-phosphorylated STAT2 associates with STAT1 and IRF9 to bind the ISRE elements in the promoters of a subset of IFN-inducible genes (ISGs). In addition to activate hundreds of ISGs, IFN alpha also represses numerous target genes but the mechanistic basis for this dual effect and transcriptional repression is largely unknown. We investigated by ChIP-chip the binding dynamics of STAT2 and "active" phospho(P)-STAT2 on 113 putative IFN alpha direct target promoters before and after IFN alpha induction in Huh7 cells and primary human hepatocytes (PHH). STAT2 is already bound to 62% of our target promoters, including most "classical" ISGs, before IFN alpha treatment. 31% of STAT2 basally bound promoters also show P-STAT2 positivity. By correlating in vivo promoter occupancy with gene expression and changes in histone methylation marks we found that: 1) STAT2 plays a role in regulating ISGs expression, independently from its phosphorylation; 2) P-STAT2 is involved in ISGs repression; 3) "activated" ISGs are marked by H3K4me1 and H3K4me3 before IFN alpha; 4) "repressed" genes are marked by H3K27me3 and histone methylation plays a dominant role in driving IFN alpha-mediated ISGs repression.
2011
Antiviral Agents; Cell Line, Tumor; Chromatin Assembly and Disassembly; Female; Hepatocytes; Histones; Humans; Interferon-Stimulated Gene Factor 3, gamma Subunit; Interferon-alpha; Methylation; Middle Aged; Phosphorylation; STAT2 Transcription Factor; Transcriptional Activation; Response Elements
01 Pubblicazione su rivista::01a Articolo in rivista
Chromatin dynamics of gene activation and repression in response to interferon alpha (IFN(alpha)) reveal new roles for phosphorylated and unphosphorylated forms of the transcription factor STAT2 / B., Testoni; C., Vollenkle; Guerrieri, Francesca; S., Gerbal Chaloin; G., Blandino; Levrero, Massimo. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 286:23(2011), pp. 20217-20227. [10.1074/jbc.m111.231068]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/438632
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