Peripheral blood mononuclear cells (PBMC) from 'responders' recently boosted with hepatitis B vaccine, were studied for synthesis in vitro of antibody to hepatitis B surface antigen (anti-HBs Ab) when stimulated with pokeweed mitogen (PWM) or HBsAg. HBsAg alone can induce an antigen-specific anti-HBs Ab response in vitro; this antibody synthesis is T cell-dependent. In some responders both allogeneic T4+ cells (in absence of PWM or HBsAg) and mixed leucocyte culture supernatants (MLC/SN) (without T cells and antigen) can help responder B cells to produce anti-Hbs Ab. Thus, in some immunized subjects, B lymphocytes involved in anti-HBs Ab synthesis are in an advanced phase of differentiation and require only non-antigen specific T cell signals (B cell growth factor or B cell differentiation factor or interleukin 2, etc) to differentiate into antibody-secreting cells. Moreover, the concentration of the antigen necessary to suppress anti-HBs Ab production induced by HBsAg was five times lower than that necessary to suppress antibody production induced by PWM. T cell help for antigen induced anti-HBs Ab could be different from T cell help for the PWM-induced anti-HBs Ab response. Moreover, the finding that the low HBsAg doses inhibiting specific response did not affect the PWM-driven anti-HBs response suggests that antigen-specific T suppressor cells could play a role in this context.
INVITRO ANTI-HBS ANTIBODY-SYNTHESIS FROM ANTI-HEPATITIS-B VACCINE RECIPIENTS / Barnaba, Vincenzo; Levrero, Massimo; G., Ruberti; A., Van Dyke; A., Perrone; A., Musca; F., Balsano. - In: CLINICAL AND EXPERIMENTAL IMMUNOLOGY. - ISSN 0009-9104. - 70:2(1987), pp. 283-288.
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|Titolo:||INVITRO ANTI-HBS ANTIBODY-SYNTHESIS FROM ANTI-HEPATITIS-B VACCINE RECIPIENTS|
|Data di pubblicazione:||1987|
|Citazione:||INVITRO ANTI-HBS ANTIBODY-SYNTHESIS FROM ANTI-HEPATITIS-B VACCINE RECIPIENTS / Barnaba, Vincenzo; Levrero, Massimo; G., Ruberti; A., Van Dyke; A., Perrone; A., Musca; F., Balsano. - In: CLINICAL AND EXPERIMENTAL IMMUNOLOGY. - ISSN 0009-9104. - 70:2(1987), pp. 283-288.|
|Appartiene alla tipologia:||01a Articolo in rivista|