All mammalian hepatitis B virus genomes contain an open reading frame X (X-ORF) of unknown function which could encode a protein of 17 kDa. Using a plasmid containing the entire X-ORF preceded by the adenovirus type 2 major late promoter and its tripartite leader sequence efficient expression of the HBV X-gene was achieved. The X protein of 17 kDa was characterized by immunoblotting and immunoprecipitated with an antiserum prepared against a X fusion protein produced in E. coli. By cell fractionation and indirect immunofluorescence the X-protein was found at least in part associated with nuclei. Human cell extracts containing the X protein have been used to screen human sera for anti-HBx antibodies. Such antibodies were detected in sera from patients with active chronic hepatitis with ongoing viral replication. The efficient expression of the HBV X protein obtained will facilitate its functional analysis. © 1990.
HEPATITIS-B VIRUS (HBV) X-GENE EXPRESSION IN HUMAN-CELLS AND ANTI-HBX ANTIBODIES DETECTION IN CHRONIC HBV INFECTION / Levrero, Massimo; O., Jean Jean; C., Balsano; H., Will; M., Perricaudet. - In: VIROLOGY. - ISSN 0042-6822. - 174:1(1990), pp. 299-304. [10.1016/0042-6822(90)90079-7]
HEPATITIS-B VIRUS (HBV) X-GENE EXPRESSION IN HUMAN-CELLS AND ANTI-HBX ANTIBODIES DETECTION IN CHRONIC HBV INFECTION
LEVRERO, Massimo;
1990
Abstract
All mammalian hepatitis B virus genomes contain an open reading frame X (X-ORF) of unknown function which could encode a protein of 17 kDa. Using a plasmid containing the entire X-ORF preceded by the adenovirus type 2 major late promoter and its tripartite leader sequence efficient expression of the HBV X-gene was achieved. The X protein of 17 kDa was characterized by immunoblotting and immunoprecipitated with an antiserum prepared against a X fusion protein produced in E. coli. By cell fractionation and indirect immunofluorescence the X-protein was found at least in part associated with nuclei. Human cell extracts containing the X protein have been used to screen human sera for anti-HBx antibodies. Such antibodies were detected in sera from patients with active chronic hepatitis with ongoing viral replication. The efficient expression of the HBV X protein obtained will facilitate its functional analysis. © 1990.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
