The products of the human hepatitis B virus (HBV) and woodchuck hepatitis B virus X genes (pXs) transactivate homologous and heterologous genes including the HBV-X and core promoters, the human immunodeficiency viruses 1 (HIV-1) and 2 (HIV-2) long terminal repeats and the beta interferon regulatory sequences. We report here that pX is also able to influence the expression of both extrachromosomal transfected c-myc regulatory sequences and endogenous c-myc gene. pX acts by increasing transcription of the c-myc gene and do not affect c-myc mRNAs stability. The presence of the first AUG of the X-ORFs is indeed necessary for the production of an active pX. The very carboxyterminus of the pX protein is dispensable for this transactivating activity and at least one domain important for its action is located between aminoacids 103 and 117. © 1991.
FULL-LENGTH AND TRUNCATED VERSIONS OF THE HEPATITIS-B VIRUS (HBV) X-PROTEIN (PX) TRANSACTIVATE THE CMYC PROTOONCOGENE AT THE TRANSCRIPTIONAL LEVEL / Clara, Balsano; Maria Laura, Avantaggiati; Gioacchino, Natoli; Elisabetta De, Marzio; Hans, Will; Michel, Perricaudet; Levrero, Massimo. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 176:3(1991), pp. 985-992. [10.1016/0006-291x(91)90379-l]
FULL-LENGTH AND TRUNCATED VERSIONS OF THE HEPATITIS-B VIRUS (HBV) X-PROTEIN (PX) TRANSACTIVATE THE CMYC PROTOONCOGENE AT THE TRANSCRIPTIONAL LEVEL
LEVRERO, Massimo
1991
Abstract
The products of the human hepatitis B virus (HBV) and woodchuck hepatitis B virus X genes (pXs) transactivate homologous and heterologous genes including the HBV-X and core promoters, the human immunodeficiency viruses 1 (HIV-1) and 2 (HIV-2) long terminal repeats and the beta interferon regulatory sequences. We report here that pX is also able to influence the expression of both extrachromosomal transfected c-myc regulatory sequences and endogenous c-myc gene. pX acts by increasing transcription of the c-myc gene and do not affect c-myc mRNAs stability. The presence of the first AUG of the X-ORFs is indeed necessary for the production of an active pX. The very carboxyterminus of the pX protein is dispensable for this transactivating activity and at least one domain important for its action is located between aminoacids 103 and 117. © 1991.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
