We have constructed recombinant adenoviruses (Ad), with functional or defective E1a genes, which harbor either the hepatitis B (HB) virus s gene encoding the HB surface antigen, as well as the pre-S2 epitopes, or the bacterial gene encoding chloramphenicol acetyltransferase (CAT) under control of the Ad major late promoter (MLP). The recombinant viruses defective for E1a (Ad.MLP.S2 and Ad.CAT), which can be efficiently propagated only on 293 cells that complement this defect, and the nondefective (Ad.MLP.S2.E1A) recombinant were used to infect a wide spectrum of cells of different origin. The yields of HBs and CAT proteins obtained with these different recombinant viruses demonstrate no real advantage to using nondefective vectors, whatever the cell type infected. The injection into chimpanzees of Ad.MLP.S2 does not elicit the production of antibodies, but can immunologically prime the animals, resulting in a partial protection against HBV challenge.

DEFECTIVE AND NONDEFECTIVE ADENOVIRUS VECTORS FOR EXPRESSING FOREIGN GENES INVITRO AND INVIVO / Levrero, Massimo; Veronique, Barban; Sylvie, Manteca; Annick, Ballay; Clara, Balsamo; Maria Laura, Avantaggiati; Gioacchino, Natoli; Huub, Skellekens; Pierre, Tiollais; Michel, Perricaudet. - In: GENE. - ISSN 0378-1119. - 101:2(1991), pp. 195-202. [10.1016/0378-1119(91)90411-4]

DEFECTIVE AND NONDEFECTIVE ADENOVIRUS VECTORS FOR EXPRESSING FOREIGN GENES INVITRO AND INVIVO

LEVRERO, Massimo;
1991

Abstract

We have constructed recombinant adenoviruses (Ad), with functional or defective E1a genes, which harbor either the hepatitis B (HB) virus s gene encoding the HB surface antigen, as well as the pre-S2 epitopes, or the bacterial gene encoding chloramphenicol acetyltransferase (CAT) under control of the Ad major late promoter (MLP). The recombinant viruses defective for E1a (Ad.MLP.S2 and Ad.CAT), which can be efficiently propagated only on 293 cells that complement this defect, and the nondefective (Ad.MLP.S2.E1A) recombinant were used to infect a wide spectrum of cells of different origin. The yields of HBs and CAT proteins obtained with these different recombinant viruses demonstrate no real advantage to using nondefective vectors, whatever the cell type infected. The injection into chimpanzees of Ad.MLP.S2 does not elicit the production of antibodies, but can immunologically prime the animals, resulting in a partial protection against HBV challenge.
1991
gene expression; major late promoter; recombinant virus
01 Pubblicazione su rivista::01a Articolo in rivista
DEFECTIVE AND NONDEFECTIVE ADENOVIRUS VECTORS FOR EXPRESSING FOREIGN GENES INVITRO AND INVIVO / Levrero, Massimo; Veronique, Barban; Sylvie, Manteca; Annick, Ballay; Clara, Balsamo; Maria Laura, Avantaggiati; Gioacchino, Natoli; Huub, Skellekens; Pierre, Tiollais; Michel, Perricaudet. - In: GENE. - ISSN 0378-1119. - 101:2(1991), pp. 195-202. [10.1016/0378-1119(91)90411-4]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/438597
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