The genome of the duck hepatitis B virus (DHBV) contains an enhancer element. This sequence, of 192 bp, is located in the 3'-terminal coding region of the DNA polymerase gene (nucleotides 2159 to 2351), upstream from the pregenomic RNA start site. This enhancer potentiates a marked increased activity from the heterologous thymidine kinase promoter in an orientation-independent manner and at a proximal, as well as at a distal, location. The DHBV enhancer activates transcription in a relatively cell-type-independent manner. Sequence homologies with the nuclear factor EF-C binding site are located in the DHBV enhancer. By using the HepG2 nuclear extracts and the DHBV enhancer as probes, a complex was observed in mobility shift assays.
IDENTIFICATION OF A STRONG ENHANCER ELEMENT UPSTREAM FROM THE PREGENOMIC RNA START SITE OF THE DUCK HEPATITIS-B VIRUS GENOME / B., Crescenzo Chaigne; J., Pillot; A., Lilienbaum; Levrero, Massimo; E., Elfassi. - In: JOURNAL OF VIROLOGY. - ISSN 0022-538X. - 65:7(1991), pp. 3882-3886.
IDENTIFICATION OF A STRONG ENHANCER ELEMENT UPSTREAM FROM THE PREGENOMIC RNA START SITE OF THE DUCK HEPATITIS-B VIRUS GENOME
LEVRERO, Massimo;
1991
Abstract
The genome of the duck hepatitis B virus (DHBV) contains an enhancer element. This sequence, of 192 bp, is located in the 3'-terminal coding region of the DNA polymerase gene (nucleotides 2159 to 2351), upstream from the pregenomic RNA start site. This enhancer potentiates a marked increased activity from the heterologous thymidine kinase promoter in an orientation-independent manner and at a proximal, as well as at a distal, location. The DHBV enhancer activates transcription in a relatively cell-type-independent manner. Sequence homologies with the nuclear factor EF-C binding site are located in the DHBV enhancer. By using the HepG2 nuclear extracts and the DHBV enhancer as probes, a complex was observed in mobility shift assays.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
