The contribution of the hepatitis B virus enhancers I and Il in the regulation of the activity of the core and the X promoters was assessed in transgenic mice. Surprisingly, despite the presence of heterologous promoters linked 5' of the X gene, the transgene expression is mostly due to core promoter (Cp) activity present in the X coding sequence. Moreover, the restriction of Cp activity to hepatic tissue required the combined action of both enhancers I and II, whereas the proximity of these two enhancers was insufficient to confer tissue specificity on Xp activity. Furthermore, the liver-specific activity of the Cp was developmentally regulated in an enhancer I-independent manner.
IN-VIVO ACTIVITY OF THE HEPATITIS-B VIRUS CORE PROMOTER - TISSUE-SPECIFICITY AND TEMPORAL REGULATION / O., Billet; G., Grimber; Levrero, Massimo; K. A., Seye; P., Briand; V., Joulin. - In: JOURNAL OF VIROLOGY. - ISSN 0022-538X. - 69:9(1995), pp. 5912-5916.
IN-VIVO ACTIVITY OF THE HEPATITIS-B VIRUS CORE PROMOTER - TISSUE-SPECIFICITY AND TEMPORAL REGULATION
LEVRERO, Massimo;
1995
Abstract
The contribution of the hepatitis B virus enhancers I and Il in the regulation of the activity of the core and the X promoters was assessed in transgenic mice. Surprisingly, despite the presence of heterologous promoters linked 5' of the X gene, the transgene expression is mostly due to core promoter (Cp) activity present in the X coding sequence. Moreover, the restriction of Cp activity to hepatic tissue required the combined action of both enhancers I and II, whereas the proximity of these two enhancers was insufficient to confer tissue specificity on Xp activity. Furthermore, the liver-specific activity of the Cp was developmentally regulated in an enhancer I-independent manner.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.