Osteonectin was immunolocalized in human fetal and calf neonatal developing bone using newly developed monoclonal antibodies. The protein was localized to the cytoplasm of osteoblasts and young osteocytes. In bone matrix, strong reactivity was found in newly laid down osteoid. Bone matrix immunoreactivity was enhanced by pretreatment of sections with proteases, possibly because of an unmasking of epitopes engaged in protein-protein interactions. Osteonectin immunoreactivity was also found in preosteoblasts in all types of human fetal osteogenesis (membranous, endochondral, subperiosteal, and mantellar (Meckel's cartilage) ossification), and in some chondrocytes of metaphyseal growth plate, possibly modulating towards an osteoblastic phenotype.

Immunohistochemical localization of osteonectin in developing human and calf bone using monoclonal antibodies / Bianco, Paolo; Silvestrini, G; Termine, Jd; Bonucci, E.. - In: CALCIFIED TISSUE INTERNATIONAL. - ISSN 0171-967X. - 43:(1988), pp. 155-161. [10.1007/BF02571313]

Immunohistochemical localization of osteonectin in developing human and calf bone using monoclonal antibodies.

BIANCO, Paolo;
1988

Abstract

Osteonectin was immunolocalized in human fetal and calf neonatal developing bone using newly developed monoclonal antibodies. The protein was localized to the cytoplasm of osteoblasts and young osteocytes. In bone matrix, strong reactivity was found in newly laid down osteoid. Bone matrix immunoreactivity was enhanced by pretreatment of sections with proteases, possibly because of an unmasking of epitopes engaged in protein-protein interactions. Osteonectin immunoreactivity was also found in preosteoblasts in all types of human fetal osteogenesis (membranous, endochondral, subperiosteal, and mantellar (Meckel's cartilage) ossification), and in some chondrocytes of metaphyseal growth plate, possibly modulating towards an osteoblastic phenotype.
1988
01 Pubblicazione su rivista::01a Articolo in rivista
Immunohistochemical localization of osteonectin in developing human and calf bone using monoclonal antibodies / Bianco, Paolo; Silvestrini, G; Termine, Jd; Bonucci, E.. - In: CALCIFIED TISSUE INTERNATIONAL. - ISSN 0171-967X. - 43:(1988), pp. 155-161. [10.1007/BF02571313]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/438282
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