Trichoderma harzianum strain T22 parasitizes and controlsmany phytopathogenic fungi. Commercially, Trichoderma spp. aremarketed as biocontrol agents and soil amendments, reducing theapplication of xenobiotic compounds in the environment andresidues in food products. We used a proteomic approach to investigatechanges in the complex mixture of cellular proteins ofT. harzianum strain T22, obtained under carbon and nitrogenstarvation and from in vitro and in vivo interactions with tomatoand several phytopathogenic fungi (Botrytis cinerea, Rhizoctoniasolani and Pythium ultimum), in order to identify proteins of potentialbiotechnology value for commercial and industrial use.When T. harzianum proteins were analysed by two-dimensionalelectrophoresis (2-DE) substantial changes in the intracellularand extracellular proteomes of the mycoparasite were observed.Comparing the 2D maps of the fungus grown in minimal mediumwith glycerol as carbon source (used as control condition) withthose obtained in inducing conditions, a lot of novel and up-regulatedproteins appeared. Differentially expressed proteins weresubjected to matrix assisted laser desorption ionisation-time offlight (MALDI-TOF) mass spectrometry (MS). The in silicoanalyses of some of the novel and up-regulated spots showed interestinghomology to hypothetical and putative proteins fromother fungal species. These include novel enzymes, such as glycosylhydrolasesand metalloprotease, proteins with conserved domainsinvolved in pathogen-host interactions, such as Ras thatregulates signal transduction pathways or LRRs that is involved inhost recognition, etc.. Work is in progress to demonstrate the roleof some of these proteins in biocontrol and ability to induce systemicresistance.
Extracellular proteome of TRICHODERMA HARZIANUM to identify proteins with biotechnological value / P., Ambrosino; Scala, Valeria; R., Marra; F., Vinale; I., Soriente; S., Ferraioli; V., Carbone; M., Ruocco; S. L., Woo; M., Lorito. - In: JOURNAL OF PLANT PATHOLOGY. - ISSN 1125-4653. - STAMPA. - 86:4(2004), pp. 307-307. (Intervento presentato al convegno S.I.Pa.V XI NATIONAL MEETING tenutosi a Milano, Italy nel September 29-October 1, 2004).
Extracellular proteome of TRICHODERMA HARZIANUM to identify proteins with biotechnological value
SCALA, VALERIA;
2004
Abstract
Trichoderma harzianum strain T22 parasitizes and controlsmany phytopathogenic fungi. Commercially, Trichoderma spp. aremarketed as biocontrol agents and soil amendments, reducing theapplication of xenobiotic compounds in the environment andresidues in food products. We used a proteomic approach to investigatechanges in the complex mixture of cellular proteins ofT. harzianum strain T22, obtained under carbon and nitrogenstarvation and from in vitro and in vivo interactions with tomatoand several phytopathogenic fungi (Botrytis cinerea, Rhizoctoniasolani and Pythium ultimum), in order to identify proteins of potentialbiotechnology value for commercial and industrial use.When T. harzianum proteins were analysed by two-dimensionalelectrophoresis (2-DE) substantial changes in the intracellularand extracellular proteomes of the mycoparasite were observed.Comparing the 2D maps of the fungus grown in minimal mediumwith glycerol as carbon source (used as control condition) withthose obtained in inducing conditions, a lot of novel and up-regulatedproteins appeared. Differentially expressed proteins weresubjected to matrix assisted laser desorption ionisation-time offlight (MALDI-TOF) mass spectrometry (MS). The in silicoanalyses of some of the novel and up-regulated spots showed interestinghomology to hypothetical and putative proteins fromother fungal species. These include novel enzymes, such as glycosylhydrolasesand metalloprotease, proteins with conserved domainsinvolved in pathogen-host interactions, such as Ras thatregulates signal transduction pathways or LRRs that is involved inhost recognition, etc.. Work is in progress to demonstrate the roleof some of these proteins in biocontrol and ability to induce systemicresistance.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.