To gain more insight into similarities of different interferon-alpha (IFN-alpha) species, we evaluated neutralization and immunoactivity of a variety of IFN preparations with various monoclonal antibodies (IFN-alpha mAb), Nine IFN-alpha mAb obtained through immunization with recombinant IFN-alpha (rmAb), lymphoblastoid IFN-alpha (LY mAb), and leukocyte IFN-alpha (LE mAb) were tested. The IFN-alpha mAb were evaluated for their ability to neutralize the antiviral activity of 11 recombinant IFN-alpha subtypes, two recombinant IFN-alpha hybrids, and lymphoblastoid and leukocyte IFN-alpha preparations. The same IFN-alpha mAb were also used in immunoblotting, and some of them were used in immunoaffinity chromatography. The results of the neutralization assay reveal that the IFN-alpha mAb significantly differ in their ability to neutralize the individual IFN-alpha species. Interestingly, none of the IFN-alpha mAb was able to neutralize all the IFN-cu species, In particular, rmAb were unable to neutralize LE-IFN-alpha or LY-IFN-alpha, whereas LE mAb and LY mAb efficiently neutralized rIFN-alpha 2. In some cases, the epitopes to which IFN-alpha mAb are directed were identified through the use of synthetic fragments of IFN-alpha 2 or by evaluating the selectivity in binding to IFN-alpha subtypes.

Antigenic characterization of recombinant, lymphoblastoid, and leukocyte IFN-alpha by monoclonal antibodies / G. C., Viscomi; Antonelli, Guido; C., Bruno; L., Scapol; F., Malavasi; A., Funaro; E., Simeoni; S., Pestka; F., De Pisa; F., Dianzani. - In: JOURNAL OF INTERFERON AND CYTOKINE RESEARCH. - ISSN 1079-9907. - STAMPA. - 19:4(1999), pp. 319-326. [10.1089/107999099314018]

Antigenic characterization of recombinant, lymphoblastoid, and leukocyte IFN-alpha by monoclonal antibodies

ANTONELLI, Guido;
1999

Abstract

To gain more insight into similarities of different interferon-alpha (IFN-alpha) species, we evaluated neutralization and immunoactivity of a variety of IFN preparations with various monoclonal antibodies (IFN-alpha mAb), Nine IFN-alpha mAb obtained through immunization with recombinant IFN-alpha (rmAb), lymphoblastoid IFN-alpha (LY mAb), and leukocyte IFN-alpha (LE mAb) were tested. The IFN-alpha mAb were evaluated for their ability to neutralize the antiviral activity of 11 recombinant IFN-alpha subtypes, two recombinant IFN-alpha hybrids, and lymphoblastoid and leukocyte IFN-alpha preparations. The same IFN-alpha mAb were also used in immunoblotting, and some of them were used in immunoaffinity chromatography. The results of the neutralization assay reveal that the IFN-alpha mAb significantly differ in their ability to neutralize the individual IFN-alpha species. Interestingly, none of the IFN-alpha mAb was able to neutralize all the IFN-cu species, In particular, rmAb were unable to neutralize LE-IFN-alpha or LY-IFN-alpha, whereas LE mAb and LY mAb efficiently neutralized rIFN-alpha 2. In some cases, the epitopes to which IFN-alpha mAb are directed were identified through the use of synthetic fragments of IFN-alpha 2 or by evaluating the selectivity in binding to IFN-alpha subtypes.
1999
01 Pubblicazione su rivista::01a Articolo in rivista
Antigenic characterization of recombinant, lymphoblastoid, and leukocyte IFN-alpha by monoclonal antibodies / G. C., Viscomi; Antonelli, Guido; C., Bruno; L., Scapol; F., Malavasi; A., Funaro; E., Simeoni; S., Pestka; F., De Pisa; F., Dianzani. - In: JOURNAL OF INTERFERON AND CYTOKINE RESEARCH. - ISSN 1079-9907. - STAMPA. - 19:4(1999), pp. 319-326. [10.1089/107999099314018]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/436423
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