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Stagonospora nodorum blotch (SNB), caused by the fungus Phaeosphaeria nodorum (E. Müll.) Hedjar. (anamorph Stagonospora nodorum (Berck.) E. Castell. & Germano) is a serious wheat disease occurring all over the world. The pathogen attacks epigeous parts of the plant, with direct damage on kernel quality causing heavy yield losses (Eyal et al., 1987). In Italy the disease has been observed in the most important cereal growing areas (Pasquini et al., 2002; Iori et al., 2003). The aim of this preliminary study was to compare the effect of Stagonospora nodorum on electrophoretic protein pattern durum wheat (cv. Simeto) according to the following tests: shrivelled kernels from artificially infected plants in field plots directly analyzed and after incubation period on water agar; finally the sound kernels of the same variety infected in laboratory and then incubated on water agar plates. An isolate of Stagonospora nodorum obtained from durum wheat was used for the spore suspension (1x106 spore ml-1) utilized for artificial inoculation. Conidial suspension was applied on the plants at ear emergence. Seed samples were harvested at maturity. Shrivelled field seeds were directly tested while other shrivelled seeds were incubated for six days on water agar plates, crushed in liquid nitrogen and freeze-dried. Moreover sterilized sound kernels were inoculated in laboratory with the aforesaid conidial suspension and incubated on water agar plates for a period of six days. These last seed samples were taken at different days, crushed in liquid nitrogen and freeze-dried. The wholemeal flour of all samples were subjected to electrophoretic analysis. Extraction of proteins were obtained according to Payne et al. (1980). Glutenins were selectively extracted following the sequential procedure of Singh et al. (1991). The 1-D electrophoresis based on procedure of Payne et al. (1980) was performed on sodium dodecyl sulphate polyacrylamide gel (SDS-PAGE). The results obtained show different electrophoretic protein patterns between field trial samples and those incubated in laboratory. The analysis of the shrivelled field samples appeared almost similar to the uninoculated control. The samples incubated on water agar plates showed no significant changes respect to the uninoculated samples until the fifth day of incubation. The sixth day of incubation loss of colour intensity and smearing of all protein bands was observed. These preliminary results suggest the possibility that the destruction of proteins in durum wheat kernels may be conditioned by various factors and/or activation (or expression) of different proteolytic enzymes.

The effects of Stagonospora nodorum on Durum wheat kernels: preliminary data on analysis of proteins. Petria Giornale di patologia delle piante. / A., Iori; G., Aureli; A., L'Aurora; Scala, Valeria. - In: PETRIA. - ISSN 1120-7698. - STAMPA. - 20:2(2010), pp. 409-410. ((Intervento presentato al convegno 13th Congress of the Mediterranean Phytopathological Union tenutosi a Rome, Italy nel 20-25 june 2010.

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