A bioassay was established using freshly prepared rabbit aortic strip to monitor the effects of various biological factors contained in rabbit serum that modulate vasoconstriction. Serotonin was shown to be a major vasoconstriction modulator in rabbit serum. A rapid procedure is described for its isolation from rabbit serum by sequential gel filtration chromatography and reversed-phase HPLC using C4, C18, and C2/C18 columns with an overall cumulative yield of about 20%. It was also noted that rabbit serum contains multiple biological factors that modulate dopamine-mediated aortic contraction other than serotonin. A potent vasoconstriction inhibitor was identified in rabbit serum which appears to be a novel regulator that mediates its effect via both α-adrenergic and serotonin receptors.
IDENTIFICATION OF MULTIPLE BIOLOGICAL FACTORS IN RABBIT SERUM THAT MODULATE DOPAMINE-MEDIATED AORTIC CONSTRICTION / M. G., Leone; Silvestrini, Bruno; Palmery, Maura; C. Y., Cheng. - In: BIOCHEMISTRY INTERNATIONAL. - ISSN 0158-5231. - STAMPA. - 26:6(1992), pp. 1079-1090.
IDENTIFICATION OF MULTIPLE BIOLOGICAL FACTORS IN RABBIT SERUM THAT MODULATE DOPAMINE-MEDIATED AORTIC CONSTRICTION
SILVESTRINI, Bruno;PALMERY, Maura;
1992
Abstract
A bioassay was established using freshly prepared rabbit aortic strip to monitor the effects of various biological factors contained in rabbit serum that modulate vasoconstriction. Serotonin was shown to be a major vasoconstriction modulator in rabbit serum. A rapid procedure is described for its isolation from rabbit serum by sequential gel filtration chromatography and reversed-phase HPLC using C4, C18, and C2/C18 columns with an overall cumulative yield of about 20%. It was also noted that rabbit serum contains multiple biological factors that modulate dopamine-mediated aortic contraction other than serotonin. A potent vasoconstriction inhibitor was identified in rabbit serum which appears to be a novel regulator that mediates its effect via both α-adrenergic and serotonin receptors.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
