Much interest has recently arisen in methods for DNA fingerprinting based on the polymerase chain reaction (PCR). Among these, the Random Amplified Polymorphic DNA (RAPD) method, developed by Williams eta]. (1), is currently receiving particular attention (2) because of its extreme simplicity and requirement for minimal amounts of genomic DNA. The basic strategy involves the PCR amplification of random fragments of genomic DNA with single or multiple (3) primers of arbitrary sequence. Polymorphism between individuals (or strains) is detected as differences between the pattern of DNA fragments amplified from the different DNAs using a given primer(s).
Reproducible DNA fingerprinting with the random amplified polymorphic DNA (RAPD) method / Micheli, Mr; Bova, R; Pascale, Esterina; D'Ambrosio, E.. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - STAMPA. - 22:(1994), pp. 1921-1922. [10.1093/nar/22.10.1921]
Reproducible DNA fingerprinting with the random amplified polymorphic DNA (RAPD) method.
PASCALE, ESTERINA;
1994
Abstract
Much interest has recently arisen in methods for DNA fingerprinting based on the polymerase chain reaction (PCR). Among these, the Random Amplified Polymorphic DNA (RAPD) method, developed by Williams eta]. (1), is currently receiving particular attention (2) because of its extreme simplicity and requirement for minimal amounts of genomic DNA. The basic strategy involves the PCR amplification of random fragments of genomic DNA with single or multiple (3) primers of arbitrary sequence. Polymorphism between individuals (or strains) is detected as differences between the pattern of DNA fragments amplified from the different DNAs using a given primer(s).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
