In the human neuroblastoma cell line SK-N-BE(2), arachidonic acid (AA), supplied in the medium at micromolar concentrations, markedly enhanced [14C]stearic acid (SA) (but not [14C]palmitic acid or [14C]oleic acid) incorporation into phosphatidylinositol (PtdIns). AA failed to stimulate [14C]SA incorporation into PtdIns precursors, namely phosphatidic acid and cytidinediphosphodiacylglycerol: furthermore, enhanced [14C]SA incorporation, brought about by exogenously administered AA, was not restricted to PtdIns tetraenoic species. When cells were pulsed for 1 h with [14C]SA (either in the presence or absence of AA) and then reincubated in AA- and [14C]SA-free medium, a marked loss of radioactivity from PtdIns was observed, that however was restricted to molecular species other than tetraenoic. These results are discussed in the light of possible mechanisms through which PtdIns achieves the 1-stearoyl-2-arachidonoyl configuration.

Arachidonic acid modulates [14C] stearic acid incorporation into phosphatitylinositol, in human neuroblastoma cells / Pacini, L; Limatola, Cristina; DE LAURENZI, V; Ricci, I; Spinedi, A.. - In: JOURNAL OF NEURO-ONCOLOGY. - ISSN 0167-594X. - STAMPA. - 31:(1997), pp. 141-146. [10.1023/A:1005766304936]

Arachidonic acid modulates [14C] stearic acid incorporation into phosphatitylinositol, in human neuroblastoma cells.

LIMATOLA, Cristina;
1997

Abstract

In the human neuroblastoma cell line SK-N-BE(2), arachidonic acid (AA), supplied in the medium at micromolar concentrations, markedly enhanced [14C]stearic acid (SA) (but not [14C]palmitic acid or [14C]oleic acid) incorporation into phosphatidylinositol (PtdIns). AA failed to stimulate [14C]SA incorporation into PtdIns precursors, namely phosphatidic acid and cytidinediphosphodiacylglycerol: furthermore, enhanced [14C]SA incorporation, brought about by exogenously administered AA, was not restricted to PtdIns tetraenoic species. When cells were pulsed for 1 h with [14C]SA (either in the presence or absence of AA) and then reincubated in AA- and [14C]SA-free medium, a marked loss of radioactivity from PtdIns was observed, that however was restricted to molecular species other than tetraenoic. These results are discussed in the light of possible mechanisms through which PtdIns achieves the 1-stearoyl-2-arachidonoyl configuration.
1997
01 Pubblicazione su rivista::01a Articolo in rivista
Arachidonic acid modulates [14C] stearic acid incorporation into phosphatitylinositol, in human neuroblastoma cells / Pacini, L; Limatola, Cristina; DE LAURENZI, V; Ricci, I; Spinedi, A.. - In: JOURNAL OF NEURO-ONCOLOGY. - ISSN 0167-594X. - STAMPA. - 31:(1997), pp. 141-146. [10.1023/A:1005766304936]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/43101
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