Abnormal proliferation signals, driven by cellular or viral oncogenes, can result in the induction of apoptosis under sub-optimal cell growth conditions. The tumor suppressor p53 plays a central role in mediating oncogene-induced apoptosis, therefore transformed cells lacking p53 are generally resistant to apoptosis-promoting treatments. In a previous work we have reported that the expression of polyomavirus large T antigen causes apoptosis in differentiating myoblasts and that this phenomenon is dependent on the onset of muscle differentiation in the absence of a correct cell cycle arrest. Here we report that polyomavirus large T increases the levels and activity of p53, but these alterations are not involved in the apoptotic mechanism. Apoptosis in polyomavirus large T-expressing myoblasts is not prevented by the expression of a p53 dominant-negative mutant nor it is increased by p53 overexpression. Moreover, forced differentiation induced through the over-expression of the muscle regulatory factor MyoD, leads to apoptosis without altering p53 function and, more significantly, even in a p53-null background. Our results indicate that apoptosis induced by the activation of muscle differentiation pathways in oncogene-expressing cells can occur in a p53-independent manner.

p53-independent apoptosis induced by muscle differentiation stimuli in polyomavirus large T-expressing myoblasts / V., Gottifredi; A., Peschiaroli; G. M., Fimia; Maione, Rossella. - In: JOURNAL OF CELL SCIENCE. - ISSN 0021-9533. - 112:14(1999), pp. 2397-2407.

p53-independent apoptosis induced by muscle differentiation stimuli in polyomavirus large T-expressing myoblasts

G. M. Fimia;MAIONE, Rossella
1999

Abstract

Abnormal proliferation signals, driven by cellular or viral oncogenes, can result in the induction of apoptosis under sub-optimal cell growth conditions. The tumor suppressor p53 plays a central role in mediating oncogene-induced apoptosis, therefore transformed cells lacking p53 are generally resistant to apoptosis-promoting treatments. In a previous work we have reported that the expression of polyomavirus large T antigen causes apoptosis in differentiating myoblasts and that this phenomenon is dependent on the onset of muscle differentiation in the absence of a correct cell cycle arrest. Here we report that polyomavirus large T increases the levels and activity of p53, but these alterations are not involved in the apoptotic mechanism. Apoptosis in polyomavirus large T-expressing myoblasts is not prevented by the expression of a p53 dominant-negative mutant nor it is increased by p53 overexpression. Moreover, forced differentiation induced through the over-expression of the muscle regulatory factor MyoD, leads to apoptosis without altering p53 function and, more significantly, even in a p53-null background. Our results indicate that apoptosis induced by the activation of muscle differentiation pathways in oncogene-expressing cells can occur in a p53-independent manner.
1999
apoptosis; muscle differentiation; myod; p53; polyomavirus large t; polyomavirus larget
01 Pubblicazione su rivista::01a Articolo in rivista
p53-independent apoptosis induced by muscle differentiation stimuli in polyomavirus large T-expressing myoblasts / V., Gottifredi; A., Peschiaroli; G. M., Fimia; Maione, Rossella. - In: JOURNAL OF CELL SCIENCE. - ISSN 0021-9533. - 112:14(1999), pp. 2397-2407.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/42619
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