Chronic alcoholism is associated with atherosclerotic cardiovascular events. Atherosclerosis is an immune-mediated inflammatory disorder involving autoimmune reactions. The humoral immune response to endothelium has a pivotal role in thè development of atherosclerosis. Autoimmune reactions are also associated with atherosclerotic alcohol-related damages but thè mecha-nisms responsible are largely unknown. Excessive ethanol consumptìon may result directly in increased oxidative stress and inflammatìon, and indirectly by thè development of autoantibodies against oxidized low density lipoprotein (ox-LDL) and phospholipids. Ali these mechanisrns may contribute to alcohol-induced injuries. Aim of this study ìs thè identificaù'on of autoantigens involved in thè pathogenesis of atherosclerotic disorders in chronic alcoholic patients. Screening of a cDNA library from human umbilical arterv endothelial cells (HUAEC) was performed with sera from two chronic alcoholic patients with carotid atherosclerosis ascertained by colour eco-doppler. Four positive strongly reactive clones were identified. The nucleotide sequence of thè cloned cDNA insertion revealed: (i) zinc finger protein 92, (ii) recombining binding protein suppressor of hairless, (iii) SWAP-70 protein. and (iv) excision repair protein. Further studies are needed to clarify thè linkage between serum antibodies to these proteins and thè atherosclerotic manifestations in chronic alcoholism. Having these recombinant antìgens might help in defming thè pre¬cise role that specific autoantibodies play in thè autoimmune mechanisms underlying atherosclerosis in chronic alcoholists.
Screening Of An Endothelial Cdna Library Identifies Several Potential Autoantigens In Chronic Alcoholics With Atherosclerotic PLAQ11E / Margutti, P; Delunardo, F; Mancinelli, R; Colasanti, T; Rigano, R; Salvati, B; Attilia, Maria Luisa; Ceccanti, Mauro; Ottona, E.. - In: ALCOHOL AND ALCOHOLISM. - ISSN 0735-0414. - STAMPA. - (2005). (Intervento presentato al convegno ESBRA 2005 tenutosi a Canterbury nel 04-07-september).
Screening Of An Endothelial Cdna Library Identifies Several Potential Autoantigens In Chronic Alcoholics With Atherosclerotic PLAQ11E
COLASANTI T;ATTILIA, Maria Luisa;CECCANTI, Mauro;
2005
Abstract
Chronic alcoholism is associated with atherosclerotic cardiovascular events. Atherosclerosis is an immune-mediated inflammatory disorder involving autoimmune reactions. The humoral immune response to endothelium has a pivotal role in thè development of atherosclerosis. Autoimmune reactions are also associated with atherosclerotic alcohol-related damages but thè mecha-nisms responsible are largely unknown. Excessive ethanol consumptìon may result directly in increased oxidative stress and inflammatìon, and indirectly by thè development of autoantibodies against oxidized low density lipoprotein (ox-LDL) and phospholipids. Ali these mechanisrns may contribute to alcohol-induced injuries. Aim of this study ìs thè identificaù'on of autoantigens involved in thè pathogenesis of atherosclerotic disorders in chronic alcoholic patients. Screening of a cDNA library from human umbilical arterv endothelial cells (HUAEC) was performed with sera from two chronic alcoholic patients with carotid atherosclerosis ascertained by colour eco-doppler. Four positive strongly reactive clones were identified. The nucleotide sequence of thè cloned cDNA insertion revealed: (i) zinc finger protein 92, (ii) recombining binding protein suppressor of hairless, (iii) SWAP-70 protein. and (iv) excision repair protein. Further studies are needed to clarify thè linkage between serum antibodies to these proteins and thè atherosclerotic manifestations in chronic alcoholism. Having these recombinant antìgens might help in defming thè pre¬cise role that specific autoantibodies play in thè autoimmune mechanisms underlying atherosclerosis in chronic alcoholists.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
