We describe a pathway by which the master transcription factor PU.1 regulates human monocyte/macrophage differentiation. This includes miR-424 and the transcriptional factor NFI-A. We show that PU.1 and these two components are interlinked in a finely tuned temporal and regulatory circuitry: PU.1 activates the transcription of miR-424, and this up-regulation is involved in stimulating monocyte differentiation through miR-424-dependent translational repression of NFI-A. In turn, the decrease in NFI-A levels is important for the activation of differentiation-specific genes such as M-CSFr. In line with these data, both RNAi against NFI-A and ectopic expression of miR-424 in precursor cells enhance monocytic differentiation, whereas the ectopic expression of NFI-A has an opposite effect. The interplay among these three components was demonstrated in myeloid cell lines as well as in human CD34+ differentiation. These data point to the important role of miR-424 and NFI-A in controlling the monocyte/macrophage differentiation program.

The interplay between the master transcription factor PU.1 and miR-424 regulates human monocyte/macrophage differentiation / Ballarino, Monica; Sorrentino, A; Sthandier, O; Marchioni, M; Masella, B; Guarini, Anna; Fatica, Alessandro; Peschle, C; Bozzoni, Irene; Rosa, Alessandro; DE ANGELIS, FERNANDA GABRIELLA. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - STAMPA. - 104:(2007), pp. 19849-19854. [10.1073/pnas.0706963104]

The interplay between the master transcription factor PU.1 and miR-424 regulates human monocyte/macrophage differentiation.

BALLARINO, MONICA;GUARINI, Anna;FATICA, Alessandro;BOZZONI, Irene;ROSA, ALESSANDRO;DE ANGELIS, FERNANDA GABRIELLA
2007

Abstract

We describe a pathway by which the master transcription factor PU.1 regulates human monocyte/macrophage differentiation. This includes miR-424 and the transcriptional factor NFI-A. We show that PU.1 and these two components are interlinked in a finely tuned temporal and regulatory circuitry: PU.1 activates the transcription of miR-424, and this up-regulation is involved in stimulating monocyte differentiation through miR-424-dependent translational repression of NFI-A. In turn, the decrease in NFI-A levels is important for the activation of differentiation-specific genes such as M-CSFr. In line with these data, both RNAi against NFI-A and ectopic expression of miR-424 in precursor cells enhance monocytic differentiation, whereas the ectopic expression of NFI-A has an opposite effect. The interplay among these three components was demonstrated in myeloid cell lines as well as in human CD34+ differentiation. These data point to the important role of miR-424 and NFI-A in controlling the monocyte/macrophage differentiation program.
2007
01 Pubblicazione su rivista::01a Articolo in rivista
The interplay between the master transcription factor PU.1 and miR-424 regulates human monocyte/macrophage differentiation / Ballarino, Monica; Sorrentino, A; Sthandier, O; Marchioni, M; Masella, B; Guarini, Anna; Fatica, Alessandro; Peschle, C; Bozzoni, Irene; Rosa, Alessandro; DE ANGELIS, FERNANDA GABRIELLA. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - STAMPA. - 104:(2007), pp. 19849-19854. [10.1073/pnas.0706963104]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/41995
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