24-25(OH)2 D3 is a vitamin D metabolite due to renal 24-hydroxylation of 25OHD3. The metabolite has some important biological functions on the bone and intestine. Its affinity to the D serum binding protein allows a competitive binding protein assay to be carried out as for 25OHD3, following a chromatographic purification step on Sephadex LH 20. Serum levels of the metabolite in 18 control subjects were found to be 2,69 +/- 1,34 ng/ml while 25OHD3 was 17,41 +/- 7,39 ng/ml and the ratio 24-25(OH)2D3 /25OHD3 in the serum was 0,15. The assay of 24-25(OH)2D3 is useful for physiological studies and for identification of vitamin D depletion states.
[Assay of serum 24,25-dihydroxycholecalciferol by a competitive protein binding method] / Coen, Giorgio; A. R., Bianchi; Ballanti, Paola; Bianchini, Gabriella; E., Bravo; Mazzaferro, Sandro; G. A., Cinotti. - In: BOLLETTINO DELLA SOCIETA' ITALIANA DI BIOLOGIA SPERIMENTALE. - ISSN 0037-8771. - 57:18(1981).
[Assay of serum 24,25-dihydroxycholecalciferol by a competitive protein binding method].
COEN, Giorgio;BALLANTI, Paola;BIANCHINI, Gabriella;MAZZAFERRO, SANDRO;
1981
Abstract
24-25(OH)2 D3 is a vitamin D metabolite due to renal 24-hydroxylation of 25OHD3. The metabolite has some important biological functions on the bone and intestine. Its affinity to the D serum binding protein allows a competitive binding protein assay to be carried out as for 25OHD3, following a chromatographic purification step on Sephadex LH 20. Serum levels of the metabolite in 18 control subjects were found to be 2,69 +/- 1,34 ng/ml while 25OHD3 was 17,41 +/- 7,39 ng/ml and the ratio 24-25(OH)2D3 /25OHD3 in the serum was 0,15. The assay of 24-25(OH)2D3 is useful for physiological studies and for identification of vitamin D depletion states.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
