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EnglishEscherichia coli has the ability to resist severe acid stress, as that encountered during transit through the host stomach, and this is instrumental to host gut colonization. The glutamate-based acid resistance (AR) system plays a major role in the protection of the cell from the deleterious effects of a high-proton-concentration environment. Structural genes of this system are gadA, gadB and gadC, which encode two glutamate decarboxylase isoforms and a glutamate/γ-aminobutyrate (GABA) antiporter, respectively. Glutamate decarboxylation leads to both proton consumption and production of GABA, a neutral compound exported via the GadC antiporter. Even though the gadA and gadBC genes are 2,1 Mb apart, their transcription is under the control of the same regulators: GadE, GadX, GadW, H-NS and RpoS. These regulators also affect the expression of 12 genes located in the acid fitness island (AFI). We have identified the GadX (GadW) binding site, a 42 bp sequence, in the regulatory regions of gadA, gadBC, slp, hdeAB, gadE and gadY. All are AFI genes, but gadBC. In my talk I will show the most recent results from in vivo and in vitro analyses aimed at fully characterizing the GadX regulon. In addition I will show biochemical data on the decarboxylase’s intracellular activation/inactivation process and on additional effectors involved in GABA export.
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| Titolo: | Glutamate-based acid resistance in Escherichia coli: biochemical and regulatory aspects |
| Autori: | |
| Data di pubblicazione: | 2010 |
| Handle: | http://hdl.handle.net/11573/417538 |
| Appartiene alla tipologia: | 04d Abstract in atti di convegno |
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