The effect of insulin on human red blood cells was investigated, both on intact cells and on isolated plasma membranes, testing the responsiveness of membrane-bound enzymes - such as (Na+-K+)-ATPase and 5'-nucleotidase - as well as the ouabain binding and ionic fluxes. It appears that insulin stimulates Na-pumping mechanisms increasing (Na+-K+)-ATPase activity through an enhanced availability of pumping sites, as can be inferred from the increased ouabain binding. The apparent unresponsiveness of fluorescence polarization parameters, following insulin treatment of isolated plasma membranes and intact cells, rules out - at present - an involvement of membrane lipid fluidity in the mechanism of action of insulin on human erythrocytes. © 1986.
INSULIN EFFECTS ON HUMAN RED-BLOOD-CELLS / P., Baldini; S., Incerpi; Pascale, Esterina; C., Rinaldi; Verna, Roberto; P., Luly. - In: MOLECULAR AND CELLULAR ENDOCRINOLOGY. - ISSN 0303-7207. - STAMPA. - 46:2(1986), pp. 93-102. [10.1016/0303-7207(86)90087-0]
INSULIN EFFECTS ON HUMAN RED-BLOOD-CELLS
PASCALE, ESTERINA;VERNA, Roberto;
1986
Abstract
The effect of insulin on human red blood cells was investigated, both on intact cells and on isolated plasma membranes, testing the responsiveness of membrane-bound enzymes - such as (Na+-K+)-ATPase and 5'-nucleotidase - as well as the ouabain binding and ionic fluxes. It appears that insulin stimulates Na-pumping mechanisms increasing (Na+-K+)-ATPase activity through an enhanced availability of pumping sites, as can be inferred from the increased ouabain binding. The apparent unresponsiveness of fluorescence polarization parameters, following insulin treatment of isolated plasma membranes and intact cells, rules out - at present - an involvement of membrane lipid fluidity in the mechanism of action of insulin on human erythrocytes. © 1986.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
