Suppression of high M(r) tropomyosins (TMs) is a common feature of transformed cells. Previous work from this laboratory has demonstrated that the isoform 1 of TM, TM1, acts as an anti-oncogene in ras-transformed murine fibroblasts. In this study, we have investigated whether TM1 is a ras-specific suppressor, or a general suppressor protein of the cellular transformation. V-src transformed fibroblasts, which express decreased TM1, were transduced with a full-length cDNA to overexpress TM1. Both the control and the transduced cells expressed v-src kinase at comparable levels. TM1 expressing (src-T1) cells grew at a lower rate in monolayer, exhibited well spread, flat morphology than the control cells. Enhanced expression of TM1 resulted in improved microfilamental architecture. More significantly, src-T1 cells completely failed to grow under anchorage independent conditions. These data demonstrate that TM1 is as an anti-oncogene of functionally diverse oncogenes, and it is a class II tumor suppressor protein.
SUPPRESSION OF SRC-INDUCED TRANSFORMED PHENOTYPE BY EXPRESSION OF TROPOMYOSIN-1 / Prasad, Gl; Masuelli, Laura; Raj, Mhg; Harindranath, N.. - In: ONCOGENE. - ISSN 0950-9232. - 18(1999), pp. 2027-2032. [10.1038/sj.onc.1202264]
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Titolo: | SUPPRESSION OF SRC-INDUCED TRANSFORMED PHENOTYPE BY EXPRESSION OF TROPOMYOSIN-1 | |
Autori: | ||
Data di pubblicazione: | 1999 | |
Rivista: | ||
Citazione: | SUPPRESSION OF SRC-INDUCED TRANSFORMED PHENOTYPE BY EXPRESSION OF TROPOMYOSIN-1 / Prasad, Gl; Masuelli, Laura; Raj, Mhg; Harindranath, N.. - In: ONCOGENE. - ISSN 0950-9232. - 18(1999), pp. 2027-2032. [10.1038/sj.onc.1202264] | |
Handle: | http://hdl.handle.net/11573/41735 | |
Appartiene alla tipologia: | 01a Articolo in rivista |