Pregnancy-specific beta 1 glycoprotein (SP1-beta) was purified from human retroplacental blood by sequential anion exchange chromatography, gel chromatography and affinity chromatography. The final preparation appeared to be electrophoretically and immunochemically pure and was in particular free from any component with alpha mobility. The preparation was used as immunogen in rabbits as well as tracer and standard for radioimmunoassay and for cross- and rocket-immunoelectrophoresis. It was shown that this radioimmunoassay procedure allowed quantitative determination of SP1-beta glycoprotein without interference by the alpha component.
Interference of an alpha-2 component in immunological determinations of pregnancy-specific beta-1 glycoprotein in serum / Pala, Alessandro; DI RUZZA, A; Curtilli, L; Strom, Roberto; Moro, Maria; Reverberi, Luigi; Carenza, Luigi. - In: CLINICA CHIMICA ACTA. - ISSN 0009-8981. - STAMPA. - 168:1(1987), pp. 33-46. [10.1016/0009-8981(87)90264-6]
Interference of an alpha-2 component in immunological determinations of pregnancy-specific beta-1 glycoprotein in serum
PALA, Alessandro;STROM, Roberto;MORO, Maria;REVERBERI, Luigi;CARENZA, Luigi
1987
Abstract
Pregnancy-specific beta 1 glycoprotein (SP1-beta) was purified from human retroplacental blood by sequential anion exchange chromatography, gel chromatography and affinity chromatography. The final preparation appeared to be electrophoretically and immunochemically pure and was in particular free from any component with alpha mobility. The preparation was used as immunogen in rabbits as well as tracer and standard for radioimmunoassay and for cross- and rocket-immunoelectrophoresis. It was shown that this radioimmunoassay procedure allowed quantitative determination of SP1-beta glycoprotein without interference by the alpha component.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
