A sensitive radioenzymatic assay for the simultaneous determination of phenylethylamine, phenylethanolamine, tyramine and octopamine in plasma samples is reported. After extraction with ethanol, the amines are subjected to enzymatic N-methylation with labelled S-adenosylmethionine, preceded in the case of phenylethylamine and of tyramine, by an enzyme-catalyzed beta-hydroxylation step. The procedure is completed by extraction with toluene containing different amounts of isoamylalcohol, followed by controlled evaporation of the solvent. A generalized and highly significant increase in the concentration of all the four amines was found in plasma samples from patients suffering from hepatic encephalopathy.

Simultaneous radioenzymatic determination of phenylethylamine, phenylethanolamine, tyramine and octopamine in plasma of patients with hepatic encephalopathy / Bozzi, Argante; Cardelli, Patrizia; Strom, Roberto; Riggio, Oliviero; ROSSI FANELLI, Filippo; Cangiano, Carlo Oreste; S., Gentile; Capocaccia, Livio. - In: CLINICAL BIOCHEMISTRY. - ISSN 0009-9120. - STAMPA. - 14:4(1981), pp. 187-190. [10.1016/s0009-9120(81)91223-6]

Simultaneous radioenzymatic determination of phenylethylamine, phenylethanolamine, tyramine and octopamine in plasma of patients with hepatic encephalopathy.

BOZZI, Argante;CARDELLI, Patrizia;STROM, Roberto;RIGGIO, Oliviero;ROSSI FANELLI, Filippo;CANGIANO, Carlo Oreste;CAPOCACCIA, Livio
1981

Abstract

A sensitive radioenzymatic assay for the simultaneous determination of phenylethylamine, phenylethanolamine, tyramine and octopamine in plasma samples is reported. After extraction with ethanol, the amines are subjected to enzymatic N-methylation with labelled S-adenosylmethionine, preceded in the case of phenylethylamine and of tyramine, by an enzyme-catalyzed beta-hydroxylation step. The procedure is completed by extraction with toluene containing different amounts of isoamylalcohol, followed by controlled evaporation of the solvent. A generalized and highly significant increase in the concentration of all the four amines was found in plasma samples from patients suffering from hepatic encephalopathy.
1981
hepatic encephalopathy; neurotransmitter agents; octopamine; phenylethylamine; tyramine
01 Pubblicazione su rivista::01a Articolo in rivista
Simultaneous radioenzymatic determination of phenylethylamine, phenylethanolamine, tyramine and octopamine in plasma of patients with hepatic encephalopathy / Bozzi, Argante; Cardelli, Patrizia; Strom, Roberto; Riggio, Oliviero; ROSSI FANELLI, Filippo; Cangiano, Carlo Oreste; S., Gentile; Capocaccia, Livio. - In: CLINICAL BIOCHEMISTRY. - ISSN 0009-9120. - STAMPA. - 14:4(1981), pp. 187-190. [10.1016/s0009-9120(81)91223-6]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/414773
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