Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat values similar to those observed with the best penicillin substrates. Cloxacillin, methicillin and imipenem were hydrolysed very slowly. Hydrolysis of azthreonam could not be detected.

Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties. Biochem J. 1988 Sep 15;254(3):885-90 / Amicosante, G; Oratore, A; Franceschini, N; Maccarrone, M; Strom, Roberto; Galleni, M; Frere, Jm. - In: BIOCHEMICAL JOURNAL. - ISSN 0264-6021. - STAMPA. - 254:3(1988), pp. 885-890.

Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties. Biochem J. 1988 Sep 15;254(3):885-90.

STROM, Roberto;
1988

Abstract

Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat values similar to those observed with the best penicillin substrates. Cloxacillin, methicillin and imipenem were hydrolysed very slowly. Hydrolysis of azthreonam could not be detected.
1988
Beta-lactamases; Cephalosporins; Isoenzymes; Penicillins
01 Pubblicazione su rivista::01a Articolo in rivista
Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties. Biochem J. 1988 Sep 15;254(3):885-90 / Amicosante, G; Oratore, A; Franceschini, N; Maccarrone, M; Strom, Roberto; Galleni, M; Frere, Jm. - In: BIOCHEMICAL JOURNAL. - ISSN 0264-6021. - STAMPA. - 254:3(1988), pp. 885-890.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/414657
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