The effect of temperature in the range from 38° to 43°C upon incorporation of labeled precursors into DNA, RNA and protein of Novikoff and Morris 5123 hepatoma cells was investigated. A striking inhibition by temperature was found in both kind of tumor cells whereas regenerating liver cells were not affected. The temperature effect was observed also in cells from a human osteosarcoma. Cell free preparations were temperature insensitive. d-glucose added to the incubation mixture caused a marked inhibition. Filipin and ethanol were found to inhibit the incorporation into tumor cells at concentrations which did not significantly affect regenerating liver cells. A possible involvement of membranes in the mechanism of heat sensitivity of tumor cells is suggested. © 1969.
The biochemical mechanism of selective heat sensitivity of cancer cells. II. Studies on nucleic acids and protein synthesis / Mondovi', Bruno; A., Finazzi Agro'; G., Rotilio; Strom, Roberto; G., Moricca; A., Rossi Fanelli. - In: EUROPEAN JOURNAL OF CANCER. - ISSN 0014-2964. - STAMPA. - 5:2(1969), pp. 137-146. [10.1016/0014-2964(69)90060-7]
The biochemical mechanism of selective heat sensitivity of cancer cells. II. Studies on nucleic acids and protein synthesis
MONDOVI', Bruno;STROM, Roberto;
1969
Abstract
The effect of temperature in the range from 38° to 43°C upon incorporation of labeled precursors into DNA, RNA and protein of Novikoff and Morris 5123 hepatoma cells was investigated. A striking inhibition by temperature was found in both kind of tumor cells whereas regenerating liver cells were not affected. The temperature effect was observed also in cells from a human osteosarcoma. Cell free preparations were temperature insensitive. d-glucose added to the incubation mixture caused a marked inhibition. Filipin and ethanol were found to inhibit the incorporation into tumor cells at concentrations which did not significantly affect regenerating liver cells. A possible involvement of membranes in the mechanism of heat sensitivity of tumor cells is suggested. © 1969.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.