We report the display of human ciliary neurotrophic factor (hCNTF), a survival factor for neuronal cells belonging to the ~-helical cytokine superfamily, on the surface of the filamentous bacteriophage fd. The hCNTF cDNA was fused to a DNA sequence encoding the C-terminal domain of pIII, a minor coat protein exposed at one end of fd. Gene fusions were cloned into a plasmid containing the ColE1 plasmid and fd origins of replication, and were packaged into phagemid particles upon superinfection with M13KO7 helper phage. The resulting fusion phage bound specifically to anti-CNTF antibodies and to the recombinant soluble CNTF s-receptor. Moreover, phage-displayed hCNTF was found to possess biological activity at concentrations comparable to those of the soluble cytokine. These results demonstrate that CNTF can be displayed on phage in a correctly folded and functionally active form. Binding of fusion phage to immobilized CNTF ~-receptor and subsequent elution at low pH resulted in affinity purification of CNTF-displaying virions. Utilization of this technology should enable the selection of high-affinity variants from libraries of CNTF mutants displayed on phage.

FUNCTIONAL PHAGE DISPLAY OF CILIARY NEUROTROPHIC FACTOR / Saggio, Isabella; Isabelle, Gloaguen; Ralph, Laufer. - In: GENE. - ISSN 0378-1119. - STAMPA. - 152:1(1995), pp. 35-39. [10.1016/0378-1119(94)00733-9]

FUNCTIONAL PHAGE DISPLAY OF CILIARY NEUROTROPHIC FACTOR

SAGGIO, Isabella;
1995

Abstract

We report the display of human ciliary neurotrophic factor (hCNTF), a survival factor for neuronal cells belonging to the ~-helical cytokine superfamily, on the surface of the filamentous bacteriophage fd. The hCNTF cDNA was fused to a DNA sequence encoding the C-terminal domain of pIII, a minor coat protein exposed at one end of fd. Gene fusions were cloned into a plasmid containing the ColE1 plasmid and fd origins of replication, and were packaged into phagemid particles upon superinfection with M13KO7 helper phage. The resulting fusion phage bound specifically to anti-CNTF antibodies and to the recombinant soluble CNTF s-receptor. Moreover, phage-displayed hCNTF was found to possess biological activity at concentrations comparable to those of the soluble cytokine. These results demonstrate that CNTF can be displayed on phage in a correctly folded and functionally active form. Binding of fusion phage to immobilized CNTF ~-receptor and subsequent elution at low pH resulted in affinity purification of CNTF-displaying virions. Utilization of this technology should enable the selection of high-affinity variants from libraries of CNTF mutants displayed on phage.
1995
fd phage; fusion protein; neuro-cytokine; phagemid; receptor binding; recombinant dna
01 Pubblicazione su rivista::01a Articolo in rivista
FUNCTIONAL PHAGE DISPLAY OF CILIARY NEUROTROPHIC FACTOR / Saggio, Isabella; Isabelle, Gloaguen; Ralph, Laufer. - In: GENE. - ISSN 0378-1119. - STAMPA. - 152:1(1995), pp. 35-39. [10.1016/0378-1119(94)00733-9]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/411186
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