This paper describes the ability of human and bovine lactoferrins (HLf; BLf), iron-binding proteins belonging to the non-immune defense system, to interfere with herpes simplex virus type 1 (HSV-1) infection. Since lactoferrins are known to bind to heparan sulphate proteoglycans and to low density lipoprotein receptor, which in turn act as binding sites for the initial interaction of HSV-1 with host cells, we tested the effect of these proteins on HSV-1 multiplication in Vero cells. Both HLf and BLf are found to be potent inhibitors of HSV-1 infection, the concentrations required to inhibit the viral cytopathic effect in Vero cells by 50% being 1.41 mu M and 0.12 mu M, respectively. HLf and BLf exerted their activity through the inhibition of adsorption of virions to the cells independently of their iron withholding property showing similar activity in the apo- and iron-saturated form. The binding of [S-35]methionine-labelled HSV-1 particles to Vero cells was strongly inhibited when BLf was added during the attachment step. BLf interacts with both Vero cell surfaces and HSV-1 particles, suggesting that the hindrance of cellular receptors and/or of viral attachment proteins may be involved in its antiviral mechanism.

Lactoferrin inhibits herpes simplex virus type 1 adsorption to Vero cells / Magda, Marchetti; Longhi, Catia; Conte, Maria Pia; Silvia, Pisani; Valenti, Piera; Seganti, Lucilla. - In: ANTIVIRAL RESEARCH. - ISSN 0166-3542. - STAMPA. - 29:2-3(1996), pp. 221-231. [10.1016/0166-3542(95)00840-3]

Lactoferrin inhibits herpes simplex virus type 1 adsorption to Vero cells

LONGHI, Catia;CONTE, Maria Pia;VALENTI, PIERA;SEGANTI, Lucilla
1996

Abstract

This paper describes the ability of human and bovine lactoferrins (HLf; BLf), iron-binding proteins belonging to the non-immune defense system, to interfere with herpes simplex virus type 1 (HSV-1) infection. Since lactoferrins are known to bind to heparan sulphate proteoglycans and to low density lipoprotein receptor, which in turn act as binding sites for the initial interaction of HSV-1 with host cells, we tested the effect of these proteins on HSV-1 multiplication in Vero cells. Both HLf and BLf are found to be potent inhibitors of HSV-1 infection, the concentrations required to inhibit the viral cytopathic effect in Vero cells by 50% being 1.41 mu M and 0.12 mu M, respectively. HLf and BLf exerted their activity through the inhibition of adsorption of virions to the cells independently of their iron withholding property showing similar activity in the apo- and iron-saturated form. The binding of [S-35]methionine-labelled HSV-1 particles to Vero cells was strongly inhibited when BLf was added during the attachment step. BLf interacts with both Vero cell surfaces and HSV-1 particles, suggesting that the hindrance of cellular receptors and/or of viral attachment proteins may be involved in its antiviral mechanism.
1996
antiviral agents; herpes simplex virus type 1 (hsv-1); lactoferrin; viral adsorption
01 Pubblicazione su rivista::01a Articolo in rivista
Lactoferrin inhibits herpes simplex virus type 1 adsorption to Vero cells / Magda, Marchetti; Longhi, Catia; Conte, Maria Pia; Silvia, Pisani; Valenti, Piera; Seganti, Lucilla. - In: ANTIVIRAL RESEARCH. - ISSN 0166-3542. - STAMPA. - 29:2-3(1996), pp. 221-231. [10.1016/0166-3542(95)00840-3]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/410470
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