Poly(ADP-ribosyl)ation (PARylation) is involved in the maintenance of genomic methylation patterns through its control of DNA methyltranferase 1 (Dnmt1) activity. Our previous findings indicated that the CCCTC binding factor/Ctcf may be an important player in key events whereby PARylation controls the unmethylated status of some CpG-rich regions. Ctcf is able to activate Poly (ADP-ribose) polymerase 1 (Parp1) which ADP-ribosylates itself and, in turn, inhibits DNA methylation via non-covalent interaction between its ADP-ribose polymers and Dnmt1. By such a mechanism, Ctcf may preserve the epigenetic pattern at promoters of important housekeeping genes. Data here reported evidence Dnmt1 as a new protein partner of Ctcf. Moreover, we show that Ctcf forms a complex with Dnmt1 and PARylated Parp1 at specific Ctcf target sequences and that PARylation is responsible for the maintenance of the unmethylated status of some Ctcf-bound CpGs. We suggest a mechanism by which Parp1, tethered and activated at specific DNA target sites by Ctcf, preserves their methylation-free status.
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|Titolo:||Ctcf marks the CpGs that are maintained non methylated by PARP activity at Igf2/H19 locus|
|Data di pubblicazione:||2010|
|Appartiene alla tipologia:||04a Atto di comunicazione a congresso|