Several wild-type virulent strains of Agrobacterium rhizogenes, belonging to both biotypes 1 and 2, were analyzed for plasmid content. All the strains were found to harbor at least one large plasmid, of a size comparable to that of A. tumefaciens Ti plasmids as determined by electron microscopy. The plasmids were characterized by restriction endonuclease finger-printing and compared for sequence homology by the Southern blot-hybridization technique. Despite the diversity of the restriction cleavage patterns, the plasmids of the various strains share rather extensive sequence homology. All of the biotype 1 organisms analyzed were shown to harbor one common plasmid. © 1981.
Fingerprinting and sequence homology of plasmids from different virulent strains of Agrobacterium rhizogenes / Costantino, Paolo; Mauro, Maria Luisa; G., Micheli; Risuleo, Gianfranco; P. J. J., Hooykaas; R., Schilperoort. - In: PLASMID. - ISSN 0147-619X. - STAMPA. - 5:2(1981), pp. 170-182. [10.1016/0147-619x(81)90018-4]
Fingerprinting and sequence homology of plasmids from different virulent strains of Agrobacterium rhizogenes
COSTANTINO, Paolo;MAURO, Maria Luisa;RISULEO, Gianfranco;
1981
Abstract
Several wild-type virulent strains of Agrobacterium rhizogenes, belonging to both biotypes 1 and 2, were analyzed for plasmid content. All the strains were found to harbor at least one large plasmid, of a size comparable to that of A. tumefaciens Ti plasmids as determined by electron microscopy. The plasmids were characterized by restriction endonuclease finger-printing and compared for sequence homology by the Southern blot-hybridization technique. Despite the diversity of the restriction cleavage patterns, the plasmids of the various strains share rather extensive sequence homology. All of the biotype 1 organisms analyzed were shown to harbor one common plasmid. © 1981.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
