A new, sensitive, specific and simple spectrophotometric method for the determination of 5-phosphoribosyl-l-pyrophosphate (PRPP) is presented. PRPP is reacted with excess hypoxanthine in the presence of hypoxanthine-guanine phosphoribosyltransferase. At the end of the reaction, PRPP concentration is measured from the extent of conversion of hypoxanthine to inositate. The concentration of the purine base is determined spectrophotometrically in the presence of xanthine oxidase.
A spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate / Salerno, Costantino; Giacomello, Alessandro; Messina, Elisa. - In: EXPERIENTIA. - ISSN 0014-4754. - STAMPA. - 35:8(1979), pp. 1016-1017.
A spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate.
SALERNO, Costantino;GIACOMELLO, Alessandro;MESSINA, ELISA
1979
Abstract
A new, sensitive, specific and simple spectrophotometric method for the determination of 5-phosphoribosyl-l-pyrophosphate (PRPP) is presented. PRPP is reacted with excess hypoxanthine in the presence of hypoxanthine-guanine phosphoribosyltransferase. At the end of the reaction, PRPP concentration is measured from the extent of conversion of hypoxanthine to inositate. The concentration of the purine base is determined spectrophotometrically in the presence of xanthine oxidase.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
