Fluorometric titration curves of erythrocyte membranes with increasing lucensomycin have a sigmoid shape. This behavior, which was not present when colloidal cholesterol suspensions were used, is, however, not peculiar to the membrane structure, being also present in cholesterol-containing phospholipid micelles. Addition of acetic acid induced or increased sigmoidicity. This behavior can either be due to a true cooperativity in binding or to different fluorescence yields of the various lucensomycin-membrane complexes. The latter hypothesis appears to be slightly favored.

The interaction of the polyene antibiotic lucensomycin with cholesterol in erythrocyte membranes and in model systems. II. Cooperative effects in erythrocyte membranes / Strom, Roberto; Crifo', Carlo; SCIOSCIA SANTORO, Antonio. - In: BIOPHYSICAL JOURNAL. - ISSN 0006-3495. - STAMPA. - 13:6(1973), pp. 581-593. [10.1016/s0006-3495(73)86008-4]

The interaction of the polyene antibiotic lucensomycin with cholesterol in erythrocyte membranes and in model systems. II. Cooperative effects in erythrocyte membranes.

STROM, Roberto;CRIFO', Carlo;SCIOSCIA SANTORO, Antonio
1973

Abstract

Fluorometric titration curves of erythrocyte membranes with increasing lucensomycin have a sigmoid shape. This behavior, which was not present when colloidal cholesterol suspensions were used, is, however, not peculiar to the membrane structure, being also present in cholesterol-containing phospholipid micelles. Addition of acetic acid induced or increased sigmoidicity. This behavior can either be due to a true cooperativity in binding or to different fluorescence yields of the various lucensomycin-membrane complexes. The latter hypothesis appears to be slightly favored.
1973
cholesterol; cooperative binding; erythrocyte membranes; polyenic antibiotics
01 Pubblicazione su rivista::01a Articolo in rivista
The interaction of the polyene antibiotic lucensomycin with cholesterol in erythrocyte membranes and in model systems. II. Cooperative effects in erythrocyte membranes / Strom, Roberto; Crifo', Carlo; SCIOSCIA SANTORO, Antonio. - In: BIOPHYSICAL JOURNAL. - ISSN 0006-3495. - STAMPA. - 13:6(1973), pp. 581-593. [10.1016/s0006-3495(73)86008-4]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/404420
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 7
  • ???jsp.display-item.citation.isi??? ND
social impact