Immunoglobulin moieties on the surface of Burkitt lymphoma and chronic lymphocytic leukaemia cells were demonstrated by immunofluorescence, agglutination and the cytotoxic effect of anti IgM and anti x serum. Using purified IgM from human serum as a reference the amount of immunoglobulin on these cells could be quantitated by their efficiency in absorbing anti-IgM and anti-x serum. The residual activity of the sera was tested in three different systems. The amount of IgM on cells of a culture line derived from a Burkitt lymphoma and on cells from a patient with chronic lymphocytic leukaemia was about 25 μg/109 cells. Cells from further cases of chronic lymphocytic leukaemia showed weak reactivity with fluorescein-labelled anti-IgM serum and carried lower but detectable amounts of IgM. Cells with accumulations of IgM structures on the cell membrane were found in normal lymphoid populations obtained from human foetus and adult bone marrow by immunofluorescence. Similar cells were also seen in mouse and rabbit lymphoid cell populations.
Surface immunoglobulin moieties on lymphoid cells / Klein, E; Eskeland, T; Inoue, M; Strom, Roberto; Johansson, B.. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - STAMPA. - 62:1(1970), pp. 133-148. [10.1016/0014-4827(79)90515-9]
Surface immunoglobulin moieties on lymphoid cells
STROM, Roberto;
1970
Abstract
Immunoglobulin moieties on the surface of Burkitt lymphoma and chronic lymphocytic leukaemia cells were demonstrated by immunofluorescence, agglutination and the cytotoxic effect of anti IgM and anti x serum. Using purified IgM from human serum as a reference the amount of immunoglobulin on these cells could be quantitated by their efficiency in absorbing anti-IgM and anti-x serum. The residual activity of the sera was tested in three different systems. The amount of IgM on cells of a culture line derived from a Burkitt lymphoma and on cells from a patient with chronic lymphocytic leukaemia was about 25 μg/109 cells. Cells from further cases of chronic lymphocytic leukaemia showed weak reactivity with fluorescein-labelled anti-IgM serum and carried lower but detectable amounts of IgM. Cells with accumulations of IgM structures on the cell membrane were found in normal lymphoid populations obtained from human foetus and adult bone marrow by immunofluorescence. Similar cells were also seen in mouse and rabbit lymphoid cell populations.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
