Objective. Systemic sclerosis (SSc) is characterised by microvascular damage due to an impairment of different angiogenic and angiostatic factors. The aim of this study was to measure plasma levels of nine molecules involved in these vascular processes in a group of SSc patients, with respect to healthy controls (NC). Methods. Sixty-five patients (M/F=2163; mean age=57.29 yrs; mean disease duration=9.63 yrs) with established SSc according to ARA criteria, and sixteen age- and sex- matched NC were enrolled. Plasma levels of vascular endothelial growth factor (VEGF), angiopoietin-2 (Ang-2), platelet derived growth factor- bb (PDGF-BB), platelet endothelial cellular adhesion molecule-1 (PECAM-I), leptin, hepathocyte growth factor (HGF), follistatin, granulocyte-colony stimulating factor (G-CSF) and interleukin 8 (IL-8) were measured using commercially available immunoassay kits (Human Angiogenesis 9-Plex Panel, Bio-Rad Laboratories). Results. We detected a significant increase of Ang-2 (median value 1315.4 mechaniSMS vs. 538.73 pg/ml p=0.0292), HGF (median value 2886.16 pg/ml vs 1296.16 pglml; p=0.0001) IL-8 (median value 32.22 pg/ml vs. 16.86 pg/ml; p=0.02), leptin (median value 32589.1 pg/mg vs. 10679.61 pg/ml; p=0.0065), PDGF-BB (median value 7258.6 pg/mg vs. 2913.44 pg/ml; p=0.000.5), PECAM-1 (median value 21681.81 pg/mg vs. 10354.53 pg/ml; p=0.0003) and VEGF (median value 236.72 pg/mg vs. 122,905 pg/mg p=0.0073) in patients with SSc with respect to NC. Higher levels of PDGF-BB (p=0.03) and PECAM-1 (p=0.05) were found in patients with digital ulcers while lower levels of PECAM-1 were found in patients with pulmonary hypertension (PH). Besides, levels of IL-8 were higher in patients with PH (p=0.04) and lower in those with pulmonary fibrosis (p=0.0.5), while levels of Ang-2 were higher in those with a "late" nailfold video-capillaroscopy (NVC) pattern with respect to those with an "early/active" one (p=0.05). Moreover, plasma levels of VEGF (p =0.02) and PDGF-BB (p=0.04) were significantly higher in those patients positive for anti-topoisomemse I antibodies. Conclusion. Our findings show significantly higher circulating levels of seven angiogenic parameters in SSc patients, thus reflecting the disregulation of endothelium in this disease. Abnormal levels of these molecules may be considered an attempt for compensatory although ineffective mechaniSMS of vascular function, leading to the development of the main clinical manifestations of SSc.

Abnormal plasma levels of different angiogenic molecules are associated with different clinical manifestations in patients with systemic sclerosis / Riccieri, Valeria; Stefanantoni, Katia; Vasile, Massimiliano; V., Macri; Sciarra, Iliana; N., Iannace; Alessandri, Cristiano; Valesini, Guido. - In: CLINICAL AND EXPERIMENTAL RHEUMATOLOGY. - ISSN 0392-856X. - STAMPA. - 29:2 (Suppl. 65)(2011), pp. S46-S52.

Abnormal plasma levels of different angiogenic molecules are associated with different clinical manifestations in patients with systemic sclerosis

RICCIERI, Valeria;STEFANANTONI, KATIA;VASILE, MASSIMILIANO;SCIARRA, ILIANA;ALESSANDRI, cristiano;VALESINI, Guido
2011

Abstract

Objective. Systemic sclerosis (SSc) is characterised by microvascular damage due to an impairment of different angiogenic and angiostatic factors. The aim of this study was to measure plasma levels of nine molecules involved in these vascular processes in a group of SSc patients, with respect to healthy controls (NC). Methods. Sixty-five patients (M/F=2163; mean age=57.29 yrs; mean disease duration=9.63 yrs) with established SSc according to ARA criteria, and sixteen age- and sex- matched NC were enrolled. Plasma levels of vascular endothelial growth factor (VEGF), angiopoietin-2 (Ang-2), platelet derived growth factor- bb (PDGF-BB), platelet endothelial cellular adhesion molecule-1 (PECAM-I), leptin, hepathocyte growth factor (HGF), follistatin, granulocyte-colony stimulating factor (G-CSF) and interleukin 8 (IL-8) were measured using commercially available immunoassay kits (Human Angiogenesis 9-Plex Panel, Bio-Rad Laboratories). Results. We detected a significant increase of Ang-2 (median value 1315.4 mechaniSMS vs. 538.73 pg/ml p=0.0292), HGF (median value 2886.16 pg/ml vs 1296.16 pglml; p=0.0001) IL-8 (median value 32.22 pg/ml vs. 16.86 pg/ml; p=0.02), leptin (median value 32589.1 pg/mg vs. 10679.61 pg/ml; p=0.0065), PDGF-BB (median value 7258.6 pg/mg vs. 2913.44 pg/ml; p=0.000.5), PECAM-1 (median value 21681.81 pg/mg vs. 10354.53 pg/ml; p=0.0003) and VEGF (median value 236.72 pg/mg vs. 122,905 pg/mg p=0.0073) in patients with SSc with respect to NC. Higher levels of PDGF-BB (p=0.03) and PECAM-1 (p=0.05) were found in patients with digital ulcers while lower levels of PECAM-1 were found in patients with pulmonary hypertension (PH). Besides, levels of IL-8 were higher in patients with PH (p=0.04) and lower in those with pulmonary fibrosis (p=0.0.5), while levels of Ang-2 were higher in those with a "late" nailfold video-capillaroscopy (NVC) pattern with respect to those with an "early/active" one (p=0.05). Moreover, plasma levels of VEGF (p =0.02) and PDGF-BB (p=0.04) were significantly higher in those patients positive for anti-topoisomemse I antibodies. Conclusion. Our findings show significantly higher circulating levels of seven angiogenic parameters in SSc patients, thus reflecting the disregulation of endothelium in this disease. Abnormal levels of these molecules may be considered an attempt for compensatory although ineffective mechaniSMS of vascular function, leading to the development of the main clinical manifestations of SSc.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/400401
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