Site-directed mutagenesis techniques have been applied to the glyA gene of E. coli coding for serine hydroxymethyltransferase, in order to substitute amino acid residues known to be at the active site of the enzyme. Two high efficient mutagenesis methods have been used that specifically select, in vivo or in vitro, mutant DNA with respect to the wild-type one.
Site-directed mutagenesis in the study of serine hydroxymethyltransferase / E., Fattori; Angelaccio, Sebastiana; Pascarella, Stefano; Bossa, Francesco; Barra, Donatella; V., Schirch. - In: ATTI DELLA ACCADEMIA NAZIONALE DEI LINCEI. RENDICONTI LINCEI. MATEMATICA E APPLICAZIONI. - ISSN 1720-0768. - STAMPA. - 1:9(1990), pp. 229-234.
Site-directed mutagenesis in the study of serine hydroxymethyltransferase
ANGELACCIO, Sebastiana;PASCARELLA, Stefano;BOSSA, Francesco;BARRA, Donatella;
1990
Abstract
Site-directed mutagenesis techniques have been applied to the glyA gene of E. coli coding for serine hydroxymethyltransferase, in order to substitute amino acid residues known to be at the active site of the enzyme. Two high efficient mutagenesis methods have been used that specifically select, in vivo or in vitro, mutant DNA with respect to the wild-type one.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
