Using monoclonal antibodies against CD2, CD4, CD8 and CD19 antigens and an automated biotin-avidin immunoperoxidase technique on whole blood samples, we evaluated the technical performance and clinical usefulness of lymphocyte subset counting by the routine hematology analyzer Technicon H*1. Statistical evaluation demonstrated excellent precision and very good correlation with the immunofluorimetric flow cytometer Ortho Spectrum III. Correlation between manual immunofluorescence at the microscope and the H*1 method was much poorer, owing to the high intrinsic imprecision of the manual method. Reference ranges obtained with the H*1 immunoperoxidase method in 44 healthy subjects closely matched those obtained with the Spectrum III. In 46 subjects with or at risk for HIV infection, we found with the H*1 method a significant decrease in CD4+ cells and in the CD4+/CD8+ cell ratio, which was progressively more marked in HIV- negative patients with lymphadenopathic syndrome, AIDS-related complex, and in patients with full-blown AIDS.

LYMPHOCYTE SUBSET COUNTING BY IMMUNOPEROXIDASE USING AN AUTOMATED ROUTINE HEMATOLOGIC ANALYZER / G., D'Onofrio; G., Menichella; G., Zini; C., Rumi; Pierelli, Luca; M., Quarantelli; P. L., Puggioni; G., Mango. - In: HAEMATOLOGICA. - ISSN 0390-6078. - 74:(1989), pp. 137-142.

LYMPHOCYTE SUBSET COUNTING BY IMMUNOPEROXIDASE USING AN AUTOMATED ROUTINE HEMATOLOGIC ANALYZER

PIERELLI, LUCA;
1989

Abstract

Using monoclonal antibodies against CD2, CD4, CD8 and CD19 antigens and an automated biotin-avidin immunoperoxidase technique on whole blood samples, we evaluated the technical performance and clinical usefulness of lymphocyte subset counting by the routine hematology analyzer Technicon H*1. Statistical evaluation demonstrated excellent precision and very good correlation with the immunofluorimetric flow cytometer Ortho Spectrum III. Correlation between manual immunofluorescence at the microscope and the H*1 method was much poorer, owing to the high intrinsic imprecision of the manual method. Reference ranges obtained with the H*1 immunoperoxidase method in 44 healthy subjects closely matched those obtained with the Spectrum III. In 46 subjects with or at risk for HIV infection, we found with the H*1 method a significant decrease in CD4+ cells and in the CD4+/CD8+ cell ratio, which was progressively more marked in HIV- negative patients with lymphadenopathic syndrome, AIDS-related complex, and in patients with full-blown AIDS.
1989
analysis; antibodies; antigens; classification/enzymology; diagnostic use; differentiation; flow cytometry; fluorescent antibody technique; hiv seropositivity; humans; immunoenzyme techniques; instrumentation; leukocyte count; lymphocytes; monoclonal; pathology; predictive value of tests
01 Pubblicazione su rivista::01a Articolo in rivista
LYMPHOCYTE SUBSET COUNTING BY IMMUNOPEROXIDASE USING AN AUTOMATED ROUTINE HEMATOLOGIC ANALYZER / G., D'Onofrio; G., Menichella; G., Zini; C., Rumi; Pierelli, Luca; M., Quarantelli; P. L., Puggioni; G., Mango. - In: HAEMATOLOGICA. - ISSN 0390-6078. - 74:(1989), pp. 137-142.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/399546
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