This paper describes the development of a semiautomated procedure for autologous bone marrow processing, prior to ex vivo manipulation and/or cryopreservation. This procedure was employed with a pediatric bowl (125 ml Latham bowl) and the automated DuPont Stericell processor. We have obtained a mononuclear cell recovery of 85% and a hemopoietic progenitor cell recovery of 81% (CFU-GM; BFU-E), with a red cell removal of 84%. We believe that a reliable and standardized bone marrow processing procedure is the basic necessity for a bone marrow transplantation program.

Semiautomated autologous bone marrow processing for transplantation / Pierelli, Luca; G., Menichella; A., Paoloni; R., Serafini; M., Vittori; M. L., Foddai; F., Meoni; P. L., Rossi; G., Leone; G., Mango. - In: HAEMATOLOGICA. - ISSN 0390-6078. - 76 Suppl 1:(1991), pp. 10-11.

Semiautomated autologous bone marrow processing for transplantation.

PIERELLI, LUCA;
1991

Abstract

This paper describes the development of a semiautomated procedure for autologous bone marrow processing, prior to ex vivo manipulation and/or cryopreservation. This procedure was employed with a pediatric bowl (125 ml Latham bowl) and the automated DuPont Stericell processor. We have obtained a mononuclear cell recovery of 85% and a hemopoietic progenitor cell recovery of 81% (CFU-GM; BFU-E), with a red cell removal of 84%. We believe that a reliable and standardized bone marrow processing procedure is the basic necessity for a bone marrow transplantation program.
1991
autologous; automation; bone marrow; bone marrow transplantation; cell count; cell separation; erythrocytes; hematopoietic stem cell transplantation; hematopoietic stem cells; humans; instrumentation; instrumentation/methods; leukocytes; methods; mononuclear; pathology; transplantation
01 Pubblicazione su rivista::01a Articolo in rivista
Semiautomated autologous bone marrow processing for transplantation / Pierelli, Luca; G., Menichella; A., Paoloni; R., Serafini; M., Vittori; M. L., Foddai; F., Meoni; P. L., Rossi; G., Leone; G., Mango. - In: HAEMATOLOGICA. - ISSN 0390-6078. - 76 Suppl 1:(1991), pp. 10-11.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/399337
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