Human gingival fibroblast primary cultures were obtained from healthy donors of different ages (20/50/65 years). The identity and homogeneity of the histotype were determined by immunofluorescence of cytoskeletal intermediate filaments (vimentin versus cytokeratin). Cell growth characteristics and doubling times, determined by growth curves, were compared in the same culture, during aging process induced in vitro, and also in cultures from donors of different ages. A progressive decrease of proliferation was found during both physiologic aging in vivo and induced aging in vitro.
[The effect of aging on the replication activity of human gingival fibroblasts in vitro] / P., Cardelli; Scarpa, Susanna; G., Zelli; E., Bove; G., Rubinacci; M., Modesti. - In: IL GIORNALE DI CHIRURGIA. - ISSN 0391-9005. - 13:3(1992).
[The effect of aging on the replication activity of human gingival fibroblasts in vitro].
SCARPA, Susanna;
1992
Abstract
Human gingival fibroblast primary cultures were obtained from healthy donors of different ages (20/50/65 years). The identity and homogeneity of the histotype were determined by immunofluorescence of cytoskeletal intermediate filaments (vimentin versus cytokeratin). Cell growth characteristics and doubling times, determined by growth curves, were compared in the same culture, during aging process induced in vitro, and also in cultures from donors of different ages. A progressive decrease of proliferation was found during both physiologic aging in vivo and induced aging in vitro.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
