The analysis of fecal neutral sterols has been improved by use of a highly selective gas-liquid chromatography column packed with SP-2401. This chromatographic column allows separation of cholesterol and cholestanol and Δ5-5α plant sterol homologs without employing silver nitrate thinlayer chromatography. Furthermore, there is no need to derivatize neutral sterols before injection. The main fecal neutral sterols are well resolved; retention times are reproducible; detector response is reproducible, linear, and sensitive to 0.2 μg. This method, successfully used for fecal samples, may be suggested as a routine method for the clinical study of cholesterol metabolism.

An improved gas liquid chromatographic method for the determination of fecal neutral sterols / Arca, Marcello; Montali, Anna; S., Ciocca; Angelico, Francesco; A., Cantafora. - In: JOURNAL OF LIPID RESEARCH. - ISSN 0022-2275. - STAMPA. - 24:3(1983), pp. 332-335.

An improved gas liquid chromatographic method for the determination of fecal neutral sterols

ARCA, Marcello;MONTALI, Anna;ANGELICO, Francesco;
1983

Abstract

The analysis of fecal neutral sterols has been improved by use of a highly selective gas-liquid chromatography column packed with SP-2401. This chromatographic column allows separation of cholesterol and cholestanol and Δ5-5α plant sterol homologs without employing silver nitrate thinlayer chromatography. Furthermore, there is no need to derivatize neutral sterols before injection. The main fecal neutral sterols are well resolved; retention times are reproducible; detector response is reproducible, linear, and sensitive to 0.2 μg. This method, successfully used for fecal samples, may be suggested as a routine method for the clinical study of cholesterol metabolism.
1983
01 Pubblicazione su rivista::01a Articolo in rivista
An improved gas liquid chromatographic method for the determination of fecal neutral sterols / Arca, Marcello; Montali, Anna; S., Ciocca; Angelico, Francesco; A., Cantafora. - In: JOURNAL OF LIPID RESEARCH. - ISSN 0022-2275. - STAMPA. - 24:3(1983), pp. 332-335.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/396789
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