The analysis of fecal neutral sterols has been improved by use of a highly selective gas-liquid chromatography column packed with SP-2401. This chromatographic column allows separation of cholesterol and cholestanol and Δ5-5α plant sterol homologs without employing silver nitrate thinlayer chromatography. Furthermore, there is no need to derivatize neutral sterols before injection. The main fecal neutral sterols are well resolved; retention times are reproducible; detector response is reproducible, linear, and sensitive to 0.2 μg. This method, successfully used for fecal samples, may be suggested as a routine method for the clinical study of cholesterol metabolism.
An improved gas liquid chromatographic method for the determination of fecal neutral sterols / Arca, Marcello; Montali, Anna; S., Ciocca; Angelico, Francesco; A., Cantafora. - In: JOURNAL OF LIPID RESEARCH. - ISSN 0022-2275. - STAMPA. - 24:3(1983), pp. 332-335.
An improved gas liquid chromatographic method for the determination of fecal neutral sterols
ARCA, Marcello;MONTALI, Anna;ANGELICO, Francesco;
1983
Abstract
The analysis of fecal neutral sterols has been improved by use of a highly selective gas-liquid chromatography column packed with SP-2401. This chromatographic column allows separation of cholesterol and cholestanol and Δ5-5α plant sterol homologs without employing silver nitrate thinlayer chromatography. Furthermore, there is no need to derivatize neutral sterols before injection. The main fecal neutral sterols are well resolved; retention times are reproducible; detector response is reproducible, linear, and sensitive to 0.2 μg. This method, successfully used for fecal samples, may be suggested as a routine method for the clinical study of cholesterol metabolism.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
