Freshwater ecosystem structure-function relationships: from theory to application

P>1. In this issue we aimed to answer the questions: (i) under what circumstances are functional variables better than structural ones for assessing ecosystem health? and (ii) are there good indicators of change in ecological functioning along perturbation gradients? 2. Of the numerous functional indicators tested in this issue, several show a response to anthropogenic stress and could be included in assessments of ecosystem health and integrity in running waters. 3. In three of eight studies, function showed a stronger response to anthropogenic stress than structure, whereas one study showed a response in structure and not function, and four studies showed responses in both structure and function. Thus structure alone could not detect all types of impairment and functional aspects should also be included and further developed for assessing running-water ecosystem health and integrity. Functional variables may be especially useful in situations where there is a stronger response among organisms not usually included in stream assessment (e.g. fungi and bacteria) than the commonly used invertebrate, macrophytes and fish indicators. 4. Leading research questions related to the use of functional indicators in running waters include: (i) how large is natural and operator-induced variation for functional indicators? (ii) how small of an effect size (delta) can be detected using structural versus functional indicators? and (iii) how do we efficiently improve theories as well as predictive ability for functional measures to assess the effects of anthropogenic stressors? 5. To advance the use of functional indicators in applied running-water studies, we need to supplement the approach of using large-scale datasets and correlation with ecosystem manipulations.