The polyether antibiotic monensin exhibited bacteriostatic activity against a clinical isolate of Legionella pneumophila in vitro. Experiments designed to test the effect of the compound on the invasiveness and multiplication of L. pneumophila in HeLa cells showed that, in the presence of the antibiotic, legionellas that penetrated the cells did not multiply. However, monensin did not alter the characteristics of phagosomes that contained ingested legionellas. In the presence of monensin, infected cells exhibited extensive vacuolation and a noticeable reduction in the number of intracellular micro-organisms was evident a few hours after infection.
EFFECT OF MONENSIN ON THE INVASIVENESS AND MULTIPLICATION OF LEGIONELLA-PNEUMOPHILA / Goldoni, Paola; M., Castellani Pastoris; L., Cattani; C., Peluso; Sinibaldi, Laura; Orsi, Nicola. - In: JOURNAL OF MEDICAL MICROBIOLOGY. - ISSN 0022-2615. - 42:4(1995), pp. 269-275. (Intervento presentato al convegno 9th meeting of the European Working Group on Legionella Infections (EWGLI) tenutosi a Viterbo nel 6-8 giugno) [10.1099/00222615-42-4-269].
EFFECT OF MONENSIN ON THE INVASIVENESS AND MULTIPLICATION OF LEGIONELLA-PNEUMOPHILA
GOLDONI, Paola;SINIBALDI, Laura;ORSI, Nicola
1995
Abstract
The polyether antibiotic monensin exhibited bacteriostatic activity against a clinical isolate of Legionella pneumophila in vitro. Experiments designed to test the effect of the compound on the invasiveness and multiplication of L. pneumophila in HeLa cells showed that, in the presence of the antibiotic, legionellas that penetrated the cells did not multiply. However, monensin did not alter the characteristics of phagosomes that contained ingested legionellas. In the presence of monensin, infected cells exhibited extensive vacuolation and a noticeable reduction in the number of intracellular micro-organisms was evident a few hours after infection.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
