The thermal response of three proteins with mesophilic, thermophilic and hyperthermophilic character hints at the essential role played in thermostability by the protein-water interface. The formation of spanning water clusters enveloping the macromolecule and their resistance to thermal stress is shown to correlate with the charge distribution at the protein surface; in particular our findings suggest an effective role of the superficial charge distribution in stabilizing the global connectivity of the hydration water.
Water around thermophilic proteins: the role of charged and apolar atoms / Fabio, Sterpone; Claudia, Bertonati; Briganti, Giuseppe; Melchionna, Simone. - In: JOURNAL OF PHYSICS. CONDENSED MATTER. - ISSN 0953-8984. - STAMPA. - 22:28(2010), p. 284113. (Intervento presentato al convegno Workshop on Modeling and Simulation of Water at Interfaces from Ambient to Supercooled Conditions. tenutosi a Lausanne, SWITZERLAND nel JUN 29-JUL 01, 2009) [10.1088/0953-8984/22/28/284113].
Water around thermophilic proteins: the role of charged and apolar atoms
BRIGANTI, Giuseppe;MELCHIONNA, SIMONE
2010
Abstract
The thermal response of three proteins with mesophilic, thermophilic and hyperthermophilic character hints at the essential role played in thermostability by the protein-water interface. The formation of spanning water clusters enveloping the macromolecule and their resistance to thermal stress is shown to correlate with the charge distribution at the protein surface; in particular our findings suggest an effective role of the superficial charge distribution in stabilizing the global connectivity of the hydration water.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
