A nuclear glycoprotein with an apparent Mr of 66,000 Da has been isolated from pig kidney chromatin after extraction with urea, guanidine-HCl and 2 M NaCl, and some of its structural features have been characterized. It belongs to the group of N-glycosylated proteins, which in the nucleus has so far received little attention. From its monosaccharide composition and recognition by lectins its oligosaccharides appear to be of high mannose and/or hybrid types. Some properties of its protein moiety suggest that it has a role in the packing of the DNA loops in the condensed chromatin.
Isolation of a novel nuclear glycoprotein from pig kidney / Eufemi, Margherita; Maras, Bruno; Valiante, S; Altieri, Fabio; Spoto, G; Turano, Carlo; Ferraro, Anna. - In: BIOCHEMISTRY INTERNATIONAL. - ISSN 0158-5231. - STAMPA. - 27:(1992), pp. 1001-1009.
Isolation of a novel nuclear glycoprotein from pig kidney.
EUFEMI, Margherita;MARAS, Bruno;ALTIERI, Fabio;TURANO, Carlo;FERRARO, Anna
1992
Abstract
A nuclear glycoprotein with an apparent Mr of 66,000 Da has been isolated from pig kidney chromatin after extraction with urea, guanidine-HCl and 2 M NaCl, and some of its structural features have been characterized. It belongs to the group of N-glycosylated proteins, which in the nucleus has so far received little attention. From its monosaccharide composition and recognition by lectins its oligosaccharides appear to be of high mannose and/or hybrid types. Some properties of its protein moiety suggest that it has a role in the packing of the DNA loops in the condensed chromatin.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
