Synthesis and properties of bioconjugates based on functionalized polymeric nanoparticles (PNs) and monoclonal anti-Integrin alpha V CD51 (aI) antibody were investigated. Polymeric and co-polymeric colloidal nanoparticles with different functionalities, i.e., acid, amine, or thiol, namely poly(methylmethacrylate-co-acrylic acid) [P(MMA-co-AA)], poly( methylmethacrylate-co-dimethylpropargylamine) [P(MMA-co-DMPA)], poly(methylmethacrylate-co-allil mercaptane) [P(MMA-co-AM)], were obtained by tailoring emulsion synthesis and fully characterized by means of spectroscopic techniques and scanning electron microscopy (SEM). Bioconjugates (PN/aI) based on P(MMA) or P(MMA-co-AA) were obtained by loading the polymeric nanoparticles with the antibody anti-lntegrin with a simple and straightforward immobilization strategy. Bioconjugates qualitative and quantitative loading analyses were carried out by means of polyacrylamide gel electrophoresis 1D-PAGE, MALDI-TOF, and LC/ESI-MS/MS investigations. The biological efficacy of bioconjugates was confirmed by the reduced migration potential of PN/al-treated human kidney cells (HEK293). The easy immobilization procedure and high immobilization capacity of polymeric nanoparticles together with tuneable chemical functionalities and dimension of the polymeric nanoparticles open applicative perspectives for targeted delivery. (C) 2011 Elsevier Inc. All rights reserved.
Nanostructured functional co-polymers bioconjugate integrin inhibitors / Lagana', Aldo; Venditti, Iole; Fratoddi, Ilaria; Capriotti, ANNA LAURA; Caruso, Giuseppe; Chiara, Battocchio; Giovanni, Polzonetti; Filippo, Acconcia; Maria, Marino; Russo, Maria Vittoria. - In: JOURNAL OF COLLOID AND INTERFACE SCIENCE. - ISSN 0021-9797. - STAMPA. - 361:2(2011), pp. 465-471. [10.1016/j.jcis.2011.05.041]
Nanostructured functional co-polymers bioconjugate integrin inhibitors
LAGANA', Aldo;VENDITTI, Iole;FRATODDI, Ilaria;CAPRIOTTI, ANNA LAURA;CARUSO, Giuseppe;RUSSO, Maria Vittoria
2011
Abstract
Synthesis and properties of bioconjugates based on functionalized polymeric nanoparticles (PNs) and monoclonal anti-Integrin alpha V CD51 (aI) antibody were investigated. Polymeric and co-polymeric colloidal nanoparticles with different functionalities, i.e., acid, amine, or thiol, namely poly(methylmethacrylate-co-acrylic acid) [P(MMA-co-AA)], poly( methylmethacrylate-co-dimethylpropargylamine) [P(MMA-co-DMPA)], poly(methylmethacrylate-co-allil mercaptane) [P(MMA-co-AM)], were obtained by tailoring emulsion synthesis and fully characterized by means of spectroscopic techniques and scanning electron microscopy (SEM). Bioconjugates (PN/aI) based on P(MMA) or P(MMA-co-AA) were obtained by loading the polymeric nanoparticles with the antibody anti-lntegrin with a simple and straightforward immobilization strategy. Bioconjugates qualitative and quantitative loading analyses were carried out by means of polyacrylamide gel electrophoresis 1D-PAGE, MALDI-TOF, and LC/ESI-MS/MS investigations. The biological efficacy of bioconjugates was confirmed by the reduced migration potential of PN/al-treated human kidney cells (HEK293). The easy immobilization procedure and high immobilization capacity of polymeric nanoparticles together with tuneable chemical functionalities and dimension of the polymeric nanoparticles open applicative perspectives for targeted delivery. (C) 2011 Elsevier Inc. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
